The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Endogenous content mapping by LM and EM ICC: 1/100. True dilution at user dilution: 1/32000. Optimal fixation: 0.1-2.5% glutaraldehyde,1% formaldehyde using HPI (High Performance Immunocytochemistry).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Gamma-aminobutyric acid (GABA) is a major inhibitory neurotransmitter. GABA acts at inhibitory synapses in the brain and spinal cord. Inhibition is provoked by GABA binding resulting in hyperpolarization of the synaptic transmembrane potential of the affected neuron. GABA binding causes ion channels to open allowing either the flow of chloride or potassium ions into or out of the cell.
Cell Membrane, Cytoplasmic, Plasma membrane and Secreted