Anti-GABA抗体(ab17413)
Key features and details
- Guinea pig polyclonal to GABA
- Suitable for: IHC-P, ICC/IF, IHC-FoFr, IHC-Fr, ELISA
- Reacts with: Species independent
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-GABA抗体
参阅全部 GABA 一抗 -
描述
豚鼠多克隆抗体to GABA -
宿主
Guinea pig -
特异性
Recognizes GABA. Staining was blocked by preabsorbing with 100uM GABA conjugated to glutaraldehyde. 500uM of similar conjugations of glutamic acid, glutamate and taurine failed to block staining. -
经测试应用
适用于: IHC-P, ICC/IF, IHC-FoFr, IHC-Fr, ELISAmore details -
种属反应性
与反应: Species independent -
免疫原
Chemical/ Small Molecule corresponding to GABA conjugated to bovine serum albumin (Glutaraldehyde).
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常规说明
Without colchicine pretreatment well stained cell bodies are visible in the cerebral cortex, cerebrallar cortex, superior colliculus and some brainstem raphe. With colchicine pretreatment, additional cell body staining is present in the interpeduncular nucleus and the dorsal column nuclei.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
Constituent: Whole serum -
Concentration information loading...
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纯度
Whole antiserum -
Primary antibody说明
Without colchine pretreatment well stained cell bodies are visible in the cerebral cortex, cerebrallar cortex, superior colliculus and some brainstem raphe. With colchine pretreatment, additional cell body staining is present in the interpeduncular nucleus and the dorsal column nuclei. -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab17413于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
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ICC/IF | (1) |
1/1000.
|
IHC-FoFr | (2) |
1/1000.
|
IHC-Fr | (2) |
1/1500.
|
ELISA |
Use at an assay dependent concentration.
|
说明 |
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IHC-P
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/1000. |
IHC-FoFr
1/1000. |
IHC-Fr
1/1500. |
ELISA
Use at an assay dependent concentration. |
靶标
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相关性
Gamma-aminobutyric acid (GABA) is a major inhibitory neurotransmitter. GABA acts at inhibitory synapses in the brain and spinal cord. Inhibition is provoked by GABA binding resulting in hyperpolarization of the synaptic transmembrane potential of the affected neuron. GABA binding causes ion channels to open allowing either the flow of chloride or potassium ions into or out of the cell. -
细胞定位
Cell Membrane, Cytoplasmic, Plasma membrane and Secreted -
别名
- 4 aminobutanoic acid antibody
- GABA antibody
- Gamma amino butyric acid antibody
- Gamma aminobutyric acid antibody
图片
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Immunohistochemical analysis of rat brain tissue, labelling GABA with ab17413 diluted 1/500. Heat-induced antigen retrieval. DAB staining. Diffuse cytoplasmic staining and axonal staining observed in ventral midbrain neurons. (Note: This antibody produces diffuse cytoplasmic staining of moderate intensity in ventral midbrain, cortical and purkinje neurons in rat brain tissue).
实验方案
数据表及文件
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Datasheet download
文献 (26)
ab17413 被引用在 26 文献中.
- Yoo YM et al. Amylin Protein Expression in the Rat Brain and Neuro-2a Cells. Int J Mol Sci 23:N/A (2022). PubMed: 35457166
- Stumpner A et al. Auditory DUM neurons in a bush-cricket: inhibited inhibitors. J Comp Physiol A Neuroethol Sens Neural Behav Phys 206:793-807 (2020). PubMed: 32656577
- Li C et al. Downregulation of microRNA-29c reduces pain after child delivery by activating the oxytocin-GABA pathway. Mol Med Rep 22:1921-1931 (2020). PubMed: 32705167
- Park SY et al. Distribution of and steroid hormone effects on calbindin-D9k in the immature rat brain. Brain Res Bull 152:225-235 (2019). PubMed: 31357009
- Leroy F et al. A circuit from hippocampal CA2 to lateral septum disinhibits social aggression. Nature 564:213-218 (2018). PubMed: 30518859