概述

  • 产品名称
  • 描述
    豚鼠多克隆抗体to GABA
  • 特异性
    Recognizes GABA. Staining was blocked by preabsorbing with 100uM GABA conjugated to glutaraldehyde. 500uM of similar conjugations of glutamic acid, glutamate and taurine failed to block staining.
  • 经测试应用
    适用于: Electron Microscopy, ICC/IF, IHC-FoFr, IHC-Fr, ELISAmore details
  • 种属反应性
    与反应: Rat
  • 免疫原

    Chemical/ Small Molecule conjugated to KLH via glutaraldehyde.

  • 常规说明

    Without colchicine pretreatment well stained cell bodies are visible in the cerebral cortex, cerebrallar cortex, superior colliculus and some brainstem raphe. With colchicine pretreatment, additional cell body staining is present in the interpeduncular nucleus and the dorsal column nuclei.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    Preservative: None
    Constituents: Whole serum
  • 纯度
    Whole antiserum
  • Primary antibody说明
    Without colchine pretreatment well stained cell bodies are visible in the cerebral cortex, cerebrallar cortex, superior colliculus and some brainstem raphe. With colchine pretreatment, additional cell body staining is present in the interpeduncular nucleus and the dorsal column nuclei.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab17413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Electron Microscopy Use at an assay dependent concentration. PubMed: 20206640
ICC/IF 1/1000.
IHC-FoFr 1/1000.
IHC-Fr 1/1500.
ELISA Use at an assay dependent concentration.

靶标

  • 相关性
    Gamma-aminobutyric acid (GABA) is a major inhibitory neurotransmitter. GABA acts at inhibitory synapses in the brain and spinal cord. Inhibition is provoked by GABA binding resulting in hyperpolarization of the synaptic transmembrane potential of the affected neuron. GABA binding causes ion channels to open allowing either the flow of chloride or potassium ions into or out of the cell.
  • 细胞定位
    Cell Membrane, Cytoplasmic, Plasma membrane and Secreted
  • 别名
    • 4 aminobutanoic acid antibody
    • GABA antibody
    • Gamma amino butyric acid antibody
    • Gamma aminobutyric acid antibody

Anti-GABA antibody 图像

  • Immunohistochemical analysis of rat brain tissue, labelling GABA with ab17413 diluted 1/500. Heat-induced antigen retrieval. DAB staining. Diffuse cytoplasmic staining and axonal staining observed in ventral midbrain neurons. (Note: This antibody produces diffuse cytoplasmic staining of moderate intensity in ventral midbrain, cortical and purkinje neurons in rat brain tissue). 

Anti-GABA antibody (ab17413)参考文献

This product has been referenced in:
  • Chen L  et al. Involvement of upregulation of miR-210 in a rat epilepsy model. Neuropsychiatr Dis Treat 12:1731-7 (2016). WB . Read more (PubMed: 27471387) »
  • Gendrel M  et al. A cellular and regulatory map of the GABAergic nervous system of C. elegans. Elife 5:N/A (2016). Caenorhabditis elegans . Read more (PubMed: 27740909) »

See all 12 Publications for this product

Product Wall

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Antigen retrieval step
None
Sample
Apteronotus leptorhynchus Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 09 2015

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (LDT brain slices)
Specification
LDT brain slices
Permeabilization
Yes - TritonX
Fixative
PFA/Gluteraldehyde
Username

Jason Teem

Verified customer

提交于 Sep 10 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Sample
Chicken Tissue sections (chicken hindbrain)
Specification
chicken hindbrain
Permeabilization
Yes - 0.1% PBS-TritonX
Fixative
Paraformaldehyde
Username

Karli Montague

Verified customer

提交于 Mar 26 2014


I can confirm that ab17413 anti-GABA antibody is sold as whole antiserum. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet. Antibody concentra...

Read More

Thank you for bringing this to our attention. We have not tested the antibody on free-floating sections but your protocol is correct, assuming 10 minutes for permeabilization of these 50um sections was sufficient. We have one other complaint about this...

Read More

Thank you for contacting us.

This antibody has been tested on GABAergic interneurons of mammalian samples, but we do not have testing or reports using hESC.

I hope this information is helpful to you. Please do not hesitate to contact ...

Read More

Thank you for providing some further useful information.

Both antibodies would be suitable for the immunostaining. However, it seems that ab17413 is more popular amongst our customers since it has been characterized in a number of application...

Read More

Thank you for contacting Abcam regarding ab17413. Regarding the tissue fixation, I have confirmed with the laboratory that yes, the combination of 4% paraformaldehyde / glutaraldehyde fixation is typically used in successful IHC staining.  Please f...

Read More

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. The details you have kindly provided will enable us to investigate this case f...

Read More

Thank you for your enquiry. I am pleased to hear this antibody has worked well for you. The is some immunogen information for this antibody is on the datasheet: Chemical / Small Molecule conjugated to KLH via glutaraldehyde. GAB...

Read More

1-10 of 12 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册