概述

  • 产品名称
    Anti-GABA抗体[5A9]
    参阅全部 GABA 一抗
  • 描述
    小鼠单克隆抗体[5A9] to GABA
  • 特异性
    ab86186 is highly specific to GABA and does not react with other amino acid BSA conjugates with the exception of weak reaction against beta alanine BSA conjugate at the highest concentrations used.
  • 经测试应用
    适用于: IHC-P, ICC/IF, IHC-FoFrmore details
  • 免疫原

    GABA coupled to BSA with glutaraldehyde

  • 常规说明


    ab86186 is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.1% Sodium Azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • 纯度
    Protein G purified
  • Primary antibody说明
    ab86186 is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.
  • 克隆
    单克隆
  • 克隆编号
    5A9
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab86186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.

靶标

  • 相关性
    Gamma-aminobutyric acid (GABA) is a major inhibitory neurotransmitter. GABA acts at inhibitory synapses in the brain and spinal cord. Inhibition is provoked by GABA binding resulting in hyperpolarization of the synaptic transmembrane potential of the affected neuron. GABA binding causes ion channels to open allowing either the flow of chloride or potassium ions into or out of the cell.
  • 细胞定位
    Cell Membrane, Cytoplasmic, Plasma membrane and Secreted
  • 别名
    • 4 aminobutanoic acid antibody
    • GABA antibody
    • Gamma amino butyric acid antibody
    • Gamma aminobutyric acid antibody

Anti-GABA antibody [5A9] 图像

  • ab86186 staining GABA in the Brains of 2-5 days old moths (Manduca) by ICC/IF (Immunocytochemistry/immunofluorescence). The head capsules were fixed with 4% paraformaldehyde, washed in PBS and embedded in Low melting point Agarose and section to 75 μm thickness.Section were blocked with 2% BSA(IgG-free) . Samples were incubated with primary antibody (1/500 in PBS).

    Double labeling against the Ms5HT1A receptor (green) and GABA (magenta) revealed a subset of Ms5HT1A-ir cell bodies co-localizing GABA (arrows). Scale bar = 100 μm.

  • Immunohistochemical analysis of human brain tissue labeling GABA with ab86186 at 1:20 dilution. Sample was incubated with primary antibody for 60 minutes.

Anti-GABA antibody [5A9] (ab86186)参考文献

This product has been referenced in:
  • Dacks AM  et al. A characterization of the Manduca sexta serotonin receptors in the context of olfactory neuromodulation. PLoS One 8:e69422 (2013). IHC-P . Read more (PubMed: 23922709) »
  • Mao YT & Pallas SL Cross-modal plasticity results in increased inhibition in primary auditory cortical areas. Neural Plast 2013:530651 (2013). IHC . Read more (PubMed: 24288625) »

See all 2 Publications for this product

Product Wall

Application
IHC - Wholemount
Sample
Drosophila melanogaster Tissue (Brain)
Specification
Brain
Username

Mr. Lee Sun

Verified customer

提交于 Oct 21 2016

GABA is a neurotransmitter and species is not applicable for this biochemical. Therefore any anti GABA antibody should work with samples of wide range of species.

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Dako Flex peroxidase as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: High pH
Sample
Human Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
10% buffered formalin
Username

Abcam user community

Verified customer

提交于 Oct 23 2014

Thank you for contacting us.

GABA is a neurotransmitter which is a small molecule; because of this species is not applicable for anti GABA antibodies - these antibodies will work irrespective of species.

I hope this information is hel...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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