Anti-GABA A receptor pi抗体(ab26055)

概述

  • 产品名称Anti-GABA A receptor pi抗体
    参阅全部 GABA A receptor pi 一抗
  • 描述
    兔多克隆抗体to GABA A receptor pi
  • 经测试应用适用于: WB, IHC-P, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Pig
    预测可用于: Cow, Dog
  • 免疫原

    Synthetic peptide derived from residues 28 - 77 of human GABA A receptor pi.

    .

  • 阳性对照
    • Jurkat cell lysate for Western blot, and Human intestine for Immunohistochemistry.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab26055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 50 kDa.Can be blocked with GABA A receptor pi peptide (ab206287). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.
ELISA 1/312500.
ICC/IF Use at an assay dependent concentration. PubMed: 22700823

靶标

  • 功能GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. In the uterus, the function of the receptor appears to be related to tissue contractility. The binding of this pI subunit with other GABA(A) receptor subunits alters the sensitivity of recombinant receptors to modulatory agents such as pregnanolone.
  • 组织特异性Most abundant in the uterus, also expressed in lung, thymus and prostate.
  • 序列相似性Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRP sub-subfamily.
  • 细胞定位Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GABA(A) receptor subunit pi antibody
    • GABRP antibody
    • Gamma aminobutyric acid (GABA) A receptor pi antibody
    • Gamma aminobutyric acid A receptor pi antibody
    • Gamma aminobutyric acid receptor pi subunit antibody
    • Gamma aminobutyric acid receptor subunit pi antibody
    • Gamma-aminobutyric acid receptor subunit pi antibody
    • GBRP_HUMAN antibody
    • MGC126386 antibody
    • MGC126387 antibody
    see all

Anti-GABA A receptor pi antibody 图像

  • Anti-GABA A receptor pi antibody (ab26055) at 0.5 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate 10ug.

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 50 kDa
    Observed band size : 50,>90 kDa (why is the actual band size different from the predicted?)
  • Ab26055 at 4-8 ug/ml staining GABA A receptor pi in paraffin embedded human intestine by Immunohistochemistry. Arrows indicate the GABA A receptor pi positive epithelial cells of intestinal villus. Magnification: x400.

Anti-GABA A receptor pi antibody (ab26055)参考文献

This product has been referenced in:
  • Takano K  et al. Characteristic expressions of GABA receptors and GABA producing/transporting molecules in rat kidney. PLoS One 9:e105835 (2014). Read more (PubMed: 25188493) »
  • Li Y  et al. A novel role of intestine epithelial GABAergic signaling in regulating intestinal fluid secretion. Am J Physiol Gastrointest Liver Physiol 303:G453-60 (2012). WB, ICC/IF ; Human, Mouse, Rat, Pig . Read more (PubMed: 22700823) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MDA-MB231 cell line)
Loading amount 20 µg
Specification MDA-MB231 cell line
Treatment 100µM GABA for 24hrs
Gel Running Conditions Non-reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Sep 13 2012

Thank you for your enquiry. Following communication with the source of this antiserum I have managed to find the protocol that was used to verify this antibody by IHC-P. I have uploaded it to our web site and it is available by clicking on the link...

Read More

Thank you for your enquiry and for completing our technical questionnaire. I am sorry to hear that you have been having difficulties with this antibody. I have examined the EXPASY database for the expression of GABA A receptor pi and it appears tha...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"