Anti-GABRD抗体[N151/3] (ab93619)
Key features and details
- Mouse monoclonal [N151/3] to GABRD
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG2a
概述
-
产品名称
Anti-GABRD抗体[N151/3]
参阅全部 GABRD 一抗 -
描述
小鼠单克隆抗体[N151/3] to GABRD -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Rat, Human
预测可用于: Cow -
免疫原
Synthetic peptide corresponding to Rat GABRD aa 15-34.
Sequence:CTQPHHGARAMNDIGDYVGSN
-
阳性对照
- Rat brain lysate
-
常规说明
The clone number has been updated from S151-3 to N151/3, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20ºC. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
-
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
N151/3 -
同种型
IgG2a -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab93619于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000. Predicted molecular weight: 51 kDa.
|
|
ICC/IF |
1/10.
|
说明 |
---|
WB
1/1000. Predicted molecular weight: 51 kDa. |
ICC/IF
1/10. |
靶标
-
功能
GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. -
疾病相关
Defects in GABRD are the cause of susceptibility to generalized epilepsy with febrile seizures plus type 5 (GEFS+5) [MIM:604233]. Generalized epilepsy with febrile seizures-plus refers to a rare familial condition with incomplete penetrance and large intrafamilial variability. Patients display febrile seizures persisting sometimes beyond the age of 6 years and/or a variety of afebrile seizure types. GEFS+ is a disease combining febrile seizures, generalized seizures often precipitated by fever at age 6 years or more, and partial seizures, with a variable degree of severity.
Defects in GABRD are the cause of susceptibility to idiopathic generalized epilepsy type 10 (IGE10) [MIM:613060]. A disorder characterized by recurring generalized seizures in the absence of detectable brain lesions and/or metabolic abnormalities. Generalized seizures arise diffusely and simultaneously from both hemispheres of the brain.
Defects in GABRD are the cause of susceptibility to juvenile myoclonic epilepsy type 7 (EJM7) [MIM:613060]. A subtype of idiopathic generalized epilepsy. Patients have afebrile seizures only, with onset in adolescence (rather than in childhood) and myoclonic jerks which usually occur after awakening and are triggered by sleep deprivation and fatigue. -
序列相似性
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRD sub-subfamily. -
细胞定位
Cell junction > synapse > postsynaptic cell membrane. Cell membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 2563 Human
- Entrez Gene: 29689 Rat
- Omim: 137163 Human
- SwissProt: O14764 Human
- SwissProt: P18506 Rat
- Unigene: 113882 Human
- Unigene: 10927 Rat
-
别名
- GABA(A) receptor subunit delta antibody
- Gabrd antibody
- Gamma aminobutyric acid GABA A receptor delta antibody
see all
图片
-
Anti-GABRD antibody [N151/3] (ab93619) at 1/1000 dilution + Rat cell line lysates at 15 µg
Secondary
Sheep Anti-Mouse IgG HRP
Predicted band size: 51 kDaBlock: 15% BSA for 30 minutes at RT
-
Cold 100% methanol for 10 minutes at -20°C fixed human HaCaT cells labeling GABRD (green) using ab93619 at 1/10 dilution in ICC/IF.
FITC Goat Anti-Mouse was used as the secondary antibody at 1/50 dilution for 1 hour at room temperature. STaining in the diffuse cytoplasm and dull nuclei is observed.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab93619 被引用在 1 文献中.
- Moldavan M et al. Diurnal properties of tonic and synaptic GABAA receptor-mediated currents in suprachiasmatic nucleus neurons. J Neurophysiol 126:637-652 (2021). PubMed: 34259044