Anti-GABA A Receptor beta 3抗体[S87-25] (ab98968)

概述

  • 产品名称Anti-GABA A Receptor beta 3抗体[S87-25]
    参阅全部 GABA A Receptor beta 3 一抗
  • 描述
    小鼠单克隆抗体[S87-25] to GABA A Receptor beta 3
  • 特异性Does not cross react with GABA A Receptor Beta 2 or Beta 1.
  • 经测试应用适用于: IHC-Fr, ICC/IF, Flow Cyt, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow, Orangutan
  • 免疫原

    Fusion protein: APMDVHNEMN EVAGSVGDTR NSAISFDNSG IQYRKQSMPK EGHGRYMGDR SIPHKKTHLR RRSS, corresponding to amino acids 370-433 of Mouse GABA A Receptor beta 3

  • 阳性对照
    • Rat brain lysate

性能

应用

Our Abpromise guarantee covers the use of ab98968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use a concentration of 0.1 - 1 µg/ml.
ICC/IF Use a concentration of 1 - 10 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P Use a concentration of 0.1 - 1 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 55 kDa. Sufficient for detection of GABA A Receptor beta 3 in 10µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

靶标

  • 功能Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel.
  • 疾病相关Epilepsy, childhood absence 5
  • 序列相似性Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRB3 sub-subfamily.
  • 细胞定位Cell junction, synapse, postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ECA5 antibody
    • GABA alpha receptor beta-2 subunit antibody
    • GABA(A) receptor subunit beta-3 antibody
    • GABAA receptor beta 3 subunit antibody
    • GABAA receptor subunit beta 3 antibody
    • GABR B3 antibody
    • Gabrb3 antibody
    • Gamma aminobutyric acid (GABA) A receptor beta 3 antibody
    • Gamma aminobutyric acid receptor subunit beta 3 antibody
    • Gamma-aminobutyric acid receptor subunit beta-3 antibody
    • GBRB3_HUMAN antibody
    • MGC9051 antibody
    see all

Anti-GABA A Receptor beta 3 antibody [S87-25] 图像

  • ab98968 staining GABA A Receptor beta 3 in Mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections).

  • Anti-GABA A Receptor beta 3 antibody [S87-25] (ab98968) at 1/1000 dilution + Rat brain membrane lysate

    Predicted band size : 55 kDa
  • Overlay histogram showing SH-SY5Y cells stained with ab98968 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab98968, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-GABA A Receptor beta 3 antibody [S87-25] (ab98968)参考文献

This product has been referenced in:
  • Konermann A  et al. Verification of ?-Amino-Butyric Acid (GABA) Signaling System Components in Periodontal Ligament Cells In Vivo and In Vitro. Cell Mol Neurobiol 36:1353-1363 (2016). Read more (PubMed: 26865191) »
  • Fatemi SH  et al. Expression of GABAA a2-, ß1- and ?-receptors are altered significantly in the lateral cerebellum of subjects with schizophrenia, major depression and bipolar disorder. Transl Psychiatry 3:e303 (2013). WB ; Human . Read more (PubMed: 24022508) »

See all 2 Publications for this product

Product Wall

According to uniprot (link below) there are four isoforms of GabaA Beta3, all of which ab98968 is capable of detecting. Isoforms 1 and 2 are ˜54 kDa, isoform 3 is 47 kDa, and isoform 4 is 45 kDa. Since GabaA Beta3 is glycosylated, I would suspect the s...

Read More

Thank you very much for your comprehension, it’s really appreciated.
Unfortunately, because we carry over 80,000 products, it isn't feasible for us to keep small sample sizes of our products, and therefore I am not able to offer a test sample of a...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"