Anti-GABA A Receptor beta 1 (phospho S434)抗体(ab51123)


  • 产品名称Anti-GABA A Receptor beta 1 (phospho S434)抗体
    参阅全部 GABA A Receptor beta 1 一抗
  • 描述
    兔多克隆抗体to GABA A Receptor beta 1 (phospho S434)
  • 特异性Ab51123 detects endogenous levels of GABA A Receptor beta 1 only when phosphorylated at serine 434.
  • 经测试应用适用于: ELISA, IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthesized phosphopeptide derived from human GABA-RB around the phosphorylation site of serine 434 (R-A-SP-Q-L).

  • 阳性对照
    • Human breast carcinoma tissue or extracts from cos7 cells.



Our Abpromise guarantee covers the use of ab51123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/40000.
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 53 kDa).


  • 功能GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
  • 序列相似性Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRB1 sub-subfamily.
  • 细胞定位Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AW061132 antibody
    • B230208N19Rik antibody
    • GABA(A) receptor beta 1 antibody
    • GABA(A) receptor subunit beta-1 antibody
    • GABA-A receptor, beta-1 polypeptide antibody
    • Gabrb-1 antibody
    • GABRB1 antibody
    • Gamma aminobutyric acid (GABA) A receptor beta 1 antibody
    • Gamma Aminobutyric Acid A Receptor Beta 1 antibody
    • Gamma Aminobutyric Acid Receptor , beta-1 antibody
    • Gamma-aminobutyric acid (GABA) A receptor, subunit beta 1 antibody
    • Gamma-aminobutyric acid receptor subunit beta-1 antibody
    • GARB1 antibody
    • GBRB1_HUMAN antibody
    see all

Anti-GABA A Receptor beta 1 (phospho S434) antibody 图像

  • This image shows paraffin-embedded human breast carcinoma tissue stained with ab51123 at a dilution of 1/100. Right hand image: tissue treated with immunising peptide; left hand image: untreated tissue
  • All lanes : Anti-GABA A Receptor beta 1 (phospho S434) antibody (ab51123)

    Lane 1 : Extracts from cos7 cells with immunising peptide
    Lane 2 : Extracts from cos7 cells with no peptide

    Predicted band size : 53 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab51123 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51123, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-GABA A Receptor beta 1 (phospho S434) antibody (ab51123)参考文献

This product has been referenced in:
  • Kim JH  et al. Distinctive genetic activity pattern of the human dental pulp between deciduous and permanent teeth. PLoS One 9:e102893 (2014). IHC-P ; Human . Read more (PubMed: 25047033) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step None
Sample Human Tissue sections (tooth)
Specification tooth
Permeabilization No
Fixative Formaldehyde

Abcam user community

Verified customer

提交于 Jul 17 2014