山羊多克隆抗体to GABA A Receptor alpha 4
(with a Cysteine residue linker), corresponding to internal sequence amino acids 349-361 of Human GABA A Receptor alpha 4 (NP_000800.2).
- Human Frontal Cortex lysate and Human Brain, Cortex tissue
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 62 kDa.
||Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA4 sub-subfamily.
Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
Information by UniProt
- GABA(A) receptor subunit alpha 4 antibody
- GABA(A) receptor subunit alpha-4 antibody
- GABR A4 antibody
Western blot - GABA A Receptor alpha 4 antibody (ab117080)
Anti-GABA A Receptor alpha 4 antibody (ab117080) at 0.1 µg/ml + Human Frontal Cortex lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 62 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GABA A Receptor alpha 4 antibody (ab117080)
Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with ab117080 followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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