Anti-GABA A Receptor alpha 1抗体(ab33299)


  • 产品名称
    Anti-GABA A Receptor alpha 1抗体
    参阅全部 GABA A Receptor alpha 1 一抗
  • 描述
    兔多克隆抗体to GABA A Receptor alpha 1
  • 宿主
  • 特异性
    This antibody is specific for the GABA A Receptor a1 subunit.
  • 经测试应用
    适用于: IHC-P, WB, IP, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Chicken, Human
    预测可用于: Cow, Cynomolgus monkey
  • 免疫原

    Synthetic peptide:


    , corresponding to N terminal amino acids 1-15 of the mature Rat GABA A Receptor alpha 1 containing a cysteine at the C terminus for linkage to a carrier protein.

  • 阳性对照
    • WB and IP: Rat brain microsomal tissue extract



Our Abpromise guarantee covers the use of ab33299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500. PubMed: 20650017
WB 1/10000. Predicted molecular weight: 52 kDa.
IP Use at an assay dependent concentration. 5ul immunoprecipitates the GABA A Receptor a1 subunit in rat brain microsomal preparation RIPA lysate.
IHC-Fr Use at an assay dependent concentration. Note: use 4% para glutaraldehyde fixed frozen sections permeabilized with Triton X100.


  • 功能
    GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
  • 疾病相关
    Defects in GABRA1 are the cause of childhood absence epilepsy type 4 (ECA4) [MIM:611136]. A subtype of idiopathic generalized epilepsy characterized by onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures may develop. Absence seizures may either remit or persist into adulthood.
    Defects in GABRA1 are the cause of juvenile myoclonic epilepsy type 5 (EJM5) [MIM:611136]. A subtype of idiopathic generalized epilepsy. Patients have afebrile seizures only, with onset in adolescence (rather than in childhood) and myoclonic jerks which usually occur after awakening and are triggered by sleep deprivation and fatigue.
  • 序列相似性
    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA1 sub-subfamily.
  • 细胞定位
    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ECA4 antibody
    • EIEE19 antibody
    • EJM antibody
    • EJM5 antibody
    • Gaba receptor alpha 1 polypeptide antibody
    • GABA(A) receptor antibody
    • GABA(A) receptor subunit alpha 1 antibody
    • GABA(A) receptor subunit alpha-1 antibody
    • GABA(A) receptor, alpha 1 antibody
    • GABRA 1 antibody
    • GABRA1 antibody
    • Gamma aminobutyric acid (GABA) A receptor alpha 1 antibody
    • Gamma aminobutyric acid A receptor alpha 1 antibody
    • Gamma aminobutyric acid receptor subunit alpha 1 antibody
    • Gamma aminobutyric acid type A receptor alpha1 subunit antibody
    • Gamma-aminobutyric acid receptor subunit alpha-1 antibody
    • GBRA1_HUMAN antibody
    see all


  • Anti-GABA A Receptor alpha 1 antibody (ab33299) at 1/10000 dilution + rat brain microsomal preparation tissue lysate at 10 µg

    Predicted band size: 52 kDa
    Observed band size: 51 kDa (why is the actual band size different from the predicted?)

    Proteins were visualized using an IgG (HRP) donkey anti-rabbit and a chemiluminescence detection system.

  • Immunohistochemistry analysis of formalin fixed paraffin embedded rat hippocampus tissue prepared using heat-induced epitope retrieval (HIER) labeling GABA A Receptor alpha 1 using ab33299 at 1/500 dilution. Secondary antibody was an anti-rabbit antibody and HRP-DAB.

  • Immunoprecipitation analysis: 1 mg of RIPA lysate of rat brain microsomes
    was immunoprecipitated with 5 uL of ab33299. Arrow indicates GABAA
    Receptor a1 (~50kD).

  • ab33299 staining GABA A Receptor alpha 1 in human retinal tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed with 4% paraformaldehyde, blocked with 10% donkey serum for 20 minutes at 24°C, then incubated with ab33299 at a 1/100 dilution for 16 hours at 4°C. The secondary antibody used was an Alexa-Fluor 488 (green) conjugated donkey anti-rabbit polyclonal, used at a 1/1000 dilution. Counterstained with Hoechst 33258 (blue).

    IPL - inner plexiform layer
    INL - inner nuclear layer
    OPL - outer plexiform layer
    ONL - outer nuclear layer
    INS - inner nuclear segments

    See Abreview


This product has been referenced in:
  • Hu D  et al. Bumetanide treatment during early development rescues maternal separation-induced susceptibility to stress. Sci Rep 7:11878 (2017). Read more (PubMed: 28928398) »
  • Zhou X  et al. Differential Modulation of GABAAand NMDA Receptors by an a7-nicotinic Acetylcholine Receptor Agonist in Chronic Glaucoma. Front Mol Neurosci 10:422 (2017). Read more (PubMed: 29326549) »

See all 12 Publications for this product


Abcam has not validated the combination of species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Human Cell (Human Cell (SH-SY5Y))
Yes - Triton X-100 (0'25%)
Human Cell (SH-SY5Y)
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

提交于 Dec 30 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Mouse Cell lysate - whole cell (Cortical)
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Dr. Martin Broadstock

Verified customer

提交于 Aug 16 2012

Merci de nous avoir contactés.
Voici les liens vers les guides et posters qui je l'espère vous seront utiles:
> DyLight® conjugated secondary antibodies :
> A Guide to Fluorochromes : http...

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I have had a chance to review each of the product which you have listed as well as our other products against the same targets. I would like to offer some recommendations.

Anti GABA A receptor alpha ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Frozen sections)
Human Tissue sections (Retina)
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C

Abcam user community

Verified customer

提交于 Sep 12 2011