antigen sequence, corresponding to amino acids 336-480 of Human GAB3.
Human Plasma, Liver and Tonsil lysates; Human liver tissue
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/250 - 1/500.
Belongs to the GAB family.
Contains 1 PH domain.
Phosphorylated on tyrosine residue(s) after macrophage colony-stimulating factor (M-CSF) receptor stimulation.
Information by UniProt
GRB2-associated binder 3 antibody
Immunocytochemistry/ Immunofluorescence - Anti-GAB3 antibody (ab121311)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab121311 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-GAB3 antibody (ab121311)
All lanes : Anti-GAB3 antibody (ab121311) at 1/250 dilution Lane 1 : RT4 cell lysate Lane 2 : U-251 MG cell lysate Lane 3 : Human Plasma Lane 4 : Human Liver lysate Lane 5 : Human Tonsil lysate Developed using the ECL technique.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAB3 antibody (ab121311)
ab121311 at 1/400 dilution staining GAB3 in Paraffin-embedded Human liver tissue by Immunohistochemistry.
This product has been referenced in:
et al. The whole-genome expression analysis of peripheral blood mononuclear cells from aspirin sensitive asthmatics versus aspirin tolerant patients and healthy donors after in vitro aspirin challenge. Respir Res 16:147 (2015).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"