概述

  • 产品名称Anti-GAB3抗体
    参阅全部 GAB3 一抗
  • 描述
    兔多克隆抗体to GAB3
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence, corresponding to amino acids 336-480 of Human GAB3.

  • 阳性对照
    • Human Plasma, Liver and Tonsil lysates; Human liver tissue

性能

应用

Our Abpromise guarantee covers the use of ab121311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

靶标

  • 序列相似性Belongs to the GAB family.
    Contains 1 PH domain.
  • 翻译后修饰Phosphorylated on tyrosine residue(s) after macrophage colony-stimulating factor (M-CSF) receptor stimulation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Gab3 antibody
    • GAB3_HUMAN antibody
    • GRB2-associated binder 3 antibody
    • GRB2-associated-binding protein 3 antibody
    • Growth factor receptor bound protein 2-associated protein 3 antibody
    see all

Anti-GAB3 antibody 图像

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab121311 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-GAB3 antibody (ab121311) at 1/250 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human Plasma
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

    Developed using the ECL technique
  • ab121311 at 1/400 dilution staining GAB3 in Paraffin-embedded Human liver tissue by Immunohistochemistry.

Anti-GAB3 antibody (ab121311)参考文献

ab121311 has not yet been referenced specifically in any publications.

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