Anti-GAB2 (phospho Y643)抗体(ab78262)
Key features and details
- Rabbit polyclonal to GAB2 (phospho Y643)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-GAB2 (phospho Y643)抗体
参阅全部 GAB2 一抗 -
描述
兔多克隆抗体to GAB2 (phospho Y643) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human GAB2 (phospho Y643).
-
阳性对照
- Extracts from Jurkat cells. Human brain tissue.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab78262于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500 - 1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
|
|
IHC-P |
1/50 - 1/100.
|
说明 |
---|
WB
1/500 - 1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa). |
IHC-P
1/50 - 1/100. |
靶标
-
功能
Adapter protein which acts downstream of several membrane receptors including cytokine, antigen, hormone, cell matrix and growth factor receptors to regulate multiple signaling pathways. Regulates osteoclast differentiation mediating the TNFRSF11A/RANK signaling. In allergic response, it plays a role in mast cells activation and degranulation through PI-3-kinase regulation. Also involved in the regulation of cell proliferation and hematopoiesis. -
序列相似性
Belongs to the GAB family.
Contains 1 PH domain. -
结构域
The SH3-binding motifs mediate interaction with SHC1 and GRB2.
The PH domain mediates phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,4-bisphosphate binding. -
翻译后修饰
Phosphorylated on tyrosine residue(s) by the thrombopoietin receptor (TPOR), stem cell factor receptor (SCFR), and T-cell and B-cell antigen receptors, gp130, IL-2R and IL-3R (By similarity). Phosphorylated upon stimulation of TNFRSF11A/RANK by TNFSF11/RANKL (By similarity). Phosphorylated upon EGF stimulation. Phosphorylated on tyrosine residues by HCK upon IL6 signaling.
Dephosphorylated by PTPN11. -
细胞定位
Cytoplasm. Cell membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 9846 Human
- Entrez Gene: 14389 Mouse
- Entrez Gene: 84477 Rat
- Omim: 606203 Human
- SwissProt: Q9UQC2 Human
- SwissProt: Q9Z1S8 Mouse
- SwissProt: Q9EQH1 Rat
- Unigene: 429434 Human
see all -
别名
- GAB 2 antibody
- Gab2 antibody
- GAB2_HUMAN antibody
see all
图片
-
All lanes : Anti-GAB2 (phospho Y643) antibody (ab78262) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells, treated
with IFN (2500U/ML, 30mins)
Lane 2 : Extracts from Jurkat cells, treated
with IFN (2500U/ML, 30mins) plus 5µg immunizing peptide
Lysates/proteins at 5 µg per lane.
Predicted band size: 74 kDa
Observed band size: 74 kDa
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab78262 at a 1/50 dilution.
Left image un-treated.
Right image treated with immunizing peptide.
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab78262 被引用在 1 文献中.
- Yim JH et al. Phosphoprotein profiles of candidate markers for early cellular responses to low-dose ?-radiation in normal human fibroblast cells. J Radiat Res 58:329-340 (2017). WB ; Human . PubMed: 28122968