重组
RabMAb

Anti-GAB2抗体[EPR2869] (ab108423)

概述

性能

应用

Our Abpromise guarantee covers the use of ab108423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 74 kDa.
ICC/IF 1/100 - 1/250.
  • 应用说明
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • 靶标

    • 功能
      Adapter protein which acts downstream of several membrane receptors including cytokine, antigen, hormone, cell matrix and growth factor receptors to regulate multiple signaling pathways. Regulates osteoclast differentiation mediating the TNFRSF11A/RANK signaling. In allergic response, it plays a role in mast cells activation and degranulation through PI-3-kinase regulation. Also involved in the regulation of cell proliferation and hematopoiesis.
    • 序列相似性
      Belongs to the GAB family.
      Contains 1 PH domain.
    • 结构域
      The SH3-binding motifs mediate interaction with SHC1 and GRB2.
      The PH domain mediates phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,4-bisphosphate binding.
    • 翻译后修饰
      Phosphorylated on tyrosine residue(s) by the thrombopoietin receptor (TPOR), stem cell factor receptor (SCFR), and T-cell and B-cell antigen receptors, gp130, IL-2R and IL-3R (By similarity). Phosphorylated upon stimulation of TNFRSF11A/RANK by TNFSF11/RANKL (By similarity). Phosphorylated upon EGF stimulation. Phosphorylated on tyrosine residues by HCK upon IL6 signaling.
      Dephosphorylated by PTPN11.
    • 细胞定位
      Cytoplasm. Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • GAB 2 antibody
      • Gab2 antibody
      • GAB2_HUMAN antibody
      • Grb 2 associated binder 2 antibody
      • GRB 2 associated binding protein 2 antibody
      • Grb2 associated binder 2 antibody
      • GRB2 associated binder 2 pp100 antibody
      • GRB2 associated binding protein 2 antibody
      • GRB2-associated binder 2 antibody
      • GRB2-associated-binding protein 2 antibody
      • Growth factor receptor bound protein 2 associated protein 2 antibody
      • Growth factor receptor bound protein 2-associated protein 2 antibody
      • KIAA0571 antibody
      • p97 antibody
      • PH domain containing adaptor molecule p97 antibody
      • pp100 antibody
      see all

    Anti-GAB2 antibody [EPR2869] 图像

    • All lanes : Anti-GAB2 antibody [EPR2869] (ab108423) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : TF-1 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 74 kDa
    • ab108423, at 1/100 dilution, staining GAB2 in SH-SY-5Y cells by Immunofluorescence.

    Anti-GAB2 antibody [EPR2869] (ab108423)参考文献

    This product has been referenced in:
    • Wang L  et al. Expression of signaling adaptor proteins predicts poor prognosis in pancreatic ductal adenocarcinoma. Diagn Pathol 12:42 (2017). Read more (PubMed: 28558797) »

    See 1 Publication for this product

    Product Wall

    Thank you for your response and your update.

    My colleague is out of office this week and she has asked me to look after her colleagues.

    We are pleased to hear that the replacement vial of ab108423 works fine. The different intensity...

    Read More

    I will investigate this further and will get back to you as soon as possible.

    Thank you for your patience.

    Hello! The blocking peptides for these antibodies have now been published in our catalog, they are ab133951 and ab133952. Please let me know if you have any further questions.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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