重组Anti-GAB2抗体[EP369Y] (ab32365)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP369Y] to GAB2
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-GAB2抗体[EP369Y]
参阅全部 GAB2 一抗 -
描述
兔单克隆抗体[EP369Y] to GAB2 -
宿主
Rabbit -
特异性
This antibody is predicted to detect the splice isoform 2 based on sequence homology. -
经测试应用
适用于: WBmore details
不适用于: ICC/IF,IHC or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human GAB2 aa 600-700. The exact sequence is proprietary.
-
阳性对照
- WB: K562 cell lysates
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP369Y -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32365于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500. Detects a band of approximately 97 kDa (predicted molecular weight: 74 kDa).
|
说明 |
---|
WB
1/500. Detects a band of approximately 97 kDa (predicted molecular weight: 74 kDa). |
靶标
-
功能
Adapter protein which acts downstream of several membrane receptors including cytokine, antigen, hormone, cell matrix and growth factor receptors to regulate multiple signaling pathways. Regulates osteoclast differentiation mediating the TNFRSF11A/RANK signaling. In allergic response, it plays a role in mast cells activation and degranulation through PI-3-kinase regulation. Also involved in the regulation of cell proliferation and hematopoiesis. -
序列相似性
Belongs to the GAB family.
Contains 1 PH domain. -
结构域
The SH3-binding motifs mediate interaction with SHC1 and GRB2.
The PH domain mediates phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,4-bisphosphate binding. -
翻译后修饰
Phosphorylated on tyrosine residue(s) by the thrombopoietin receptor (TPOR), stem cell factor receptor (SCFR), and T-cell and B-cell antigen receptors, gp130, IL-2R and IL-3R (By similarity). Phosphorylated upon stimulation of TNFRSF11A/RANK by TNFSF11/RANKL (By similarity). Phosphorylated upon EGF stimulation. Phosphorylated on tyrosine residues by HCK upon IL6 signaling.
Dephosphorylated by PTPN11. -
细胞定位
Cytoplasm. Cell membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 9846 Human
- Entrez Gene: 14389 Mouse
- Entrez Gene: 84477 Rat
- Omim: 606203 Human
- SwissProt: Q9UQC2 Human
- SwissProt: Q9Z1S8 Mouse
- SwissProt: Q9EQH1 Rat
- Unigene: 429434 Human
see all -
别名
- GAB 2 antibody
- Gab2 antibody
- GAB2_HUMAN antibody
see all
图片
-
All lanes : Anti-GAB2 antibody [EP369Y] (ab32365) at 1/500 dilution
Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) cell lysate, prepared in RIPA lysis method
Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) cell lysate, prepared in 1% SDS Hot lysis method
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 97 kDa why is the actual band size different from the predicted?We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.
Blocking/Diluting Buffer and concentration:
5% NFDM/TBST
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (4)
ab32365 被引用在 4 文献中.
- Sun J et al. Anti-allergic actions of a Chinese patent medicine, huoxiangzhengqi oral liquid, in RBL-2H3 cells and in mice. Pharm Biol 59:672-682 (2021). PubMed: 34078224
- Mu L et al. MicroRNA-218 inhibits tumor angiogenesis of human renal cell carcinoma by targeting GAB2. Oncol Rep 44:1961-1970 (2020). PubMed: 32901879
- Tian LQ et al. MicroRNA-197 inhibits cell proliferation by targeting GAB2 in glioblastoma. Mol Med Rep N/A:N/A (2016). WB . PubMed: 27035789
- Pyarajan S et al. Interleukin-3 (IL-3)-induced c-fos Activation Is Modulated by Gab2-Calcineurin Interaction. J Biol Chem 283:23505-9 (2008). WB . PubMed: 18586679