Anti-GAB1 (phospho Y627)抗体(ab131458)
Key features and details
- Rabbit polyclonal to GAB1 (phospho Y627)
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-GAB1 (phospho Y627)抗体
参阅全部 GAB1 一抗 -
描述
兔多克隆抗体to GAB1 (phospho Y627) -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human GAB1 (phospho Y627).
Database link: Q13480 -
阳性对照
- Methanol fixed HeLa cells; HUVEC cell extracts EGF treated.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Antibodies were purified by using epitope specific phospho peptide. Non-phospho specific antibodies were removed by chromatography using non-phospho peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131458于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500 - 1/1000. Predicted molecular weight: 77 kDa.
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|
ICC/IF |
1/100 - 1/200.
|
说明 |
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WB
1/500 - 1/1000. Predicted molecular weight: 77 kDa. |
ICC/IF
1/100 - 1/200. |
靶标
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功能
Probably involved in EGF and insulin receptor signaling. -
序列相似性
Belongs to the GAB family.
Contains 1 PH domain. -
翻译后修饰
Phosphorylated on tyrosine residue(s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains. - Information by UniProt
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数据库链接
- Entrez Gene: 2549 Human
- Omim: 604439 Human
- SwissProt: Q13480 Human
- Unigene: 618456 Human
- Unigene: 80720 Human
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别名
- Gab 1 antibody
- GAB1 antibody
- GAB1_HUMAN antibody
see all
图片
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Immunofluorescence staining of methanol fixed HeLa cells labelling GAB1 (phospho Y627) with ab131458 antibody at a dilution of 1/100.
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All lanes : Anti-GAB1 (phospho Y627) antibody (ab131458) at 1/500 dilution
Lane 1 : HUVEC cell extracts untreated
Lane 2 : HUVEC cell extracts EGF treated
Predicted band size: 77 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab131458 尚未被引用在任何文献中。