概述

  • 产品名称Anti-GAB1 (phospho Y627)抗体
    参阅全部 GAB1 一抗
  • 描述
    兔多克隆抗体to GAB1 (phospho Y627)
  • 特异性Does not cross react with Gab1 at tyrosines 242, 437, 472 or 476.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Human
  • 免疫原

    Synthetic peptide (Human).

  • 阳性对照
    • NIH3T3 cells +/- PDGF.

性能

应用

Our Abpromise guarantee covers the use of ab12863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 115 kDa.

靶标

  • 功能Probably involved in EGF and insulin receptor signaling.
  • 序列相似性Belongs to the GAB family.
    Contains 1 PH domain.
  • 翻译后修饰Phosphorylated on tyrosine residue(s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Gab 1 antibody
    • GAB1 antibody
    • GAB1_HUMAN antibody
    • GRB 2 associated binder 1 antibody
    • GRB 2 associated binding protein 1 antibody
    • GRB2 associated binding protein 1 isoform a antibody
    • GRB2 associated binding protein 1 isoform b antibody
    • GRB2-associated binder 1 antibody
    • GRB2-associated-binding protein 1 antibody
    • Growth factor receptor bound protein 2-associated protein 1 antibody
    see all

Anti-GAB1 (phospho Y627) antibody 图像

  • Peptide Competition Lysates prepared from NIH3T3 cells left untreated (1) or treated with PDGF (2-5). were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature and incubated with ab12863 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphosotyrosine containing peptide (4), the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal method. The data show that only the peptide corresponding to ab12863 blocks the signal, verifying the specificity of the antibody.

Anti-GAB1 (phospho Y627) antibody (ab12863)参考文献

This product has been referenced in:
  • Mood K  et al. Gab1 is required for cell cycle transition, cell proliferation, and transformation induced by an oncogenic met receptor. Mol Biol Cell 17:3717-28 (2006). Read more (PubMed: 16775003) »

See 1 Publication for this product

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