概述

  • 产品名称
  • 描述
    兔多克隆抗体to GAB1
  • 特异性
    ab59362 detects endogenous levels of total GAB1 protein
  • 经测试应用
    适用于: ICC/IF, WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthesized non phosphopeptide derived from human GAB1 around the phosphorylation site of tyrosine 659 (VDYPVV).

  • 阳性对照
    • NIH/3T3 cytoplasmic lysate (WB) Human breast carcinoma tissue (IHC)
  • 常规说明
    Store at -20°C for 1 year

性能

应用

Our Abpromise guarantee covers the use of ab59362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 77 kDa.
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.

靶标

  • 功能
    Probably involved in EGF and insulin receptor signaling.
  • 序列相似性
    Belongs to the GAB family.
    Contains 1 PH domain.
  • 翻译后修饰
    Phosphorylated on tyrosine residue(s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Gab 1 antibody
    • GAB1 antibody
    • GAB1_HUMAN antibody
    • GRB 2 associated binder 1 antibody
    • GRB 2 associated binding protein 1 antibody
    • GRB2 associated binding protein 1 isoform a antibody
    • GRB2 associated binding protein 1 isoform b antibody
    • GRB2-associated binder 1 antibody
    • GRB2-associated-binding protein 1 antibody
    • Growth factor receptor bound protein 2-associated protein 1 antibody
    see all

图片

  • All lanes : Anti-GAB1 antibody (ab59362) at 1/500 dilution

    Lane 1 : Extracts from NIH/3T3 cells with no immunizing peptide
    Lane 2 : Extracts from NIH/3T3 cells with immunizing peptide


    Observed band size : 77 kDa (why is the actual band size different from the predicted?)
  • ab59362, at a 1/50 dilution, staining human GAB1 in breast carcinoma, using Immunohistochemistry, Paraffin embedded tissue, in the absence of the immunizing peptide (left image) and in the presence of the immunizing peptide (right image).
  • ICC/IF image of ab59362 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59362, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

This product has been referenced in:
  • Zhang Y  et al. Identification of GRB2 and GAB1 coexpression as an unfavorable prognostic factor for hepatocellular carcinoma by a combination of expression profile and network analysis. PLoS One 8:e85170 (2013). IHC . Read more (PubMed: 24391994) »

See 1 Publication for this product

客户评价及客户问答

Please find attached the paper that we were talking about on the phone. If there is anything else I can help you with, please let me know.

Thank you for contacting us. The product was tested on  NIH/3T3 cells lysate. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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