概述

  • 产品名称Anti-GAB1抗体
    参阅全部 GAB1 一抗
  • 描述
    兔多克隆抗体to GAB1
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 644-694 of Human GAB1 (NP_002030.2).

  • 阳性对照
    • HeLa and 293T whole cell lysates.

性能

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应用

Our Abpromise guarantee covers the use of ab117774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Predicted molecular weight: 77 kDa.
IP Use at 2-10 µg/mg of lysate.

靶标

  • 功能Probably involved in EGF and insulin receptor signaling.
  • 序列相似性Belongs to the GAB family.
    Contains 1 PH domain.
  • 翻译后修饰Phosphorylated on tyrosine residue(s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Gab 1 antibody
    • GAB1 antibody
    • GAB1_HUMAN antibody
    • GRB 2 associated binder 1 antibody
    • GRB 2 associated binding protein 1 antibody
    • GRB2 associated binding protein 1 isoform a antibody
    • GRB2 associated binding protein 1 isoform b antibody
    • GRB2-associated binder 1 antibody
    • GRB2-associated-binding protein 1 antibody
    • Growth factor receptor bound protein 2-associated protein 1 antibody
    see all

Anti-GAB1 antibody 图像

  • All lanes : Anti-GAB1 antibody (ab117774) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 77 kDa


    Exposure time : 3 minutes
  • Detection of GAB1 by Western Blot of Immunprecipitate.
    ab117774 at 1µg/ml staining GAB1 in HeLa whole cell lysate immunoprecipitated using ab117774 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

Anti-GAB1 antibody (ab117774)参考文献

ab117774 has not yet been referenced specifically in any publications.

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