Anti-G3BP (phospho S232)抗体(ab38463)

概述

  • 产品名称Anti-G3BP (phospho S232)抗体
    参阅全部 G3BP 一抗
  • 描述
    兔多克隆抗体to G3BP (phospho S232)
  • 特异性This antibody is specific for G3BP only when phosphorylated at serine 232.
  • 经测试应用适用于: WB, IHC-P, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic phosphopeptide derived from Human G3BP around the phosphorylation site of serine 232.

  • 阳性对照
    • Human breast tissue slides and 293 cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab38463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 52 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/1000.
ICC/IF Use a concentration of 1 - 5 µg/ml.

靶标

  • 功能May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.
  • 组织特异性Ubiquitous.
  • 序列相似性Contains 1 NTF2 domain.
    Contains 1 RRM (RNA recognition motif) domain.
  • 结构域The NTF2 domain mediates multimerization.
  • 翻译后修饰Phosphorylated exclusively on serine residues. Hyperphosphorylated in quiescent fibroblasts. Hypophosphorylation leads to a decrease in endoribonuclease activity (By similarity). RASA1-dependent phosphorylation of Ser-149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. Ser-149 phosphorylation induces partial nuclear localization.
    Arg-435 is dimethylated, probably to asymmetric dimethylarginine.
  • 细胞定位Cytoplasm. Cytoplasm > cytosol. Cell membrane. Nucleus. Cytoplasmic in proliferating cells, can be recruited to the plasma membrane in exponentially growing cells (By similarity). Cytosolic and partially nuclear in resting cells. Recruited to stress granules (SGs) upon either arsenite or high temperature treatment. Recruitment to SGs is influenced by HRAS.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATP dependent DNA helicase VIII antibody
    • ATP-dependent DNA helicase VIII antibody
    • G3BP antibody
    • G3BP-1 antibody
    • G3bp1 antibody
    • G3BP1_HUMAN antibody
    • GAP binding protein antibody
    • GAP SH3 domain binding protein 1 antibody
    • GAP SH3 domain-binding protein 1 antibody
    • GTPase activating protein (SH3 domain) binding protein 1 antibody
    • hDH VIII antibody
    • Human DNA helicase VIII antibody
    • MGC111040 antibody
    • Ras GTPase activating protein binding protein 1 antibody
    • Ras GTPase activating protein SH3 domain binding protein antibody
    • Ras GTPase-activating protein-binding protein 1 antibody
    • RasGAP associated endoribonuclease G3BP antibody
    see all

Anti-G3BP (phospho S232) antibody 图像

  • ab38463 at a 1:50 dilution staining G3BP in Human breast carcinoma tissue using Immunohistochemistry, Paraffin Embedded Tissue.

    Left image without phosphopeptide.

    Right image with phosphopeptide.

  • All lanes : Anti-G3BP (phospho S232) antibody (ab38463) at 1/500 dilution

    Lane 1 : 293 cell extract.
    Lane 2 : 293 cell extract. Pre-incubated with synthesized phosphopeptide.


    Predicted band size : 52 kDa
  • ICC/IF image of ab38463 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38463, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-G3BP (phospho S232) antibody (ab38463)参考文献

ab38463 has not yet been referenced specifically in any publications.

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