概述

  • 产品名称Anti-G3BP抗体
    参阅全部 G3BP 一抗
  • 描述
    小鼠单克隆抗体to G3BP
  • 经测试应用适用于: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: KPEPVLEETA PEDAQKSSSP APADIAQTVQ EDLRTFSWAS VTSKNLPPSG AVPVTGIPPH VVKVPASQPR PESKPESQIP PQRPQRDQRV , corresponding to amino acids 214-303 of Human G3BP

性能

应用

Our Abpromise guarantee covers the use of ab56574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 52 kDa.
IHC-P Use a concentration of 1 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.
  • 组织特异性Ubiquitous.
  • 序列相似性Contains 1 NTF2 domain.
    Contains 1 RRM (RNA recognition motif) domain.
  • 结构域The NTF2 domain mediates multimerization.
  • 翻译后修饰Phosphorylated exclusively on serine residues. Hyperphosphorylated in quiescent fibroblasts. Hypophosphorylation leads to a decrease in endoribonuclease activity (By similarity). RASA1-dependent phosphorylation of Ser-149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. Ser-149 phosphorylation induces partial nuclear localization.
    Arg-435 is dimethylated, probably to asymmetric dimethylarginine.
  • 细胞定位Cytoplasm. Cytoplasm > cytosol. Cell membrane. Nucleus. Cytoplasmic in proliferating cells, can be recruited to the plasma membrane in exponentially growing cells (By similarity). Cytosolic and partially nuclear in resting cells. Recruited to stress granules (SGs) upon either arsenite or high temperature treatment. Recruitment to SGs is influenced by HRAS.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATP dependent DNA helicase VIII antibody
    • ATP-dependent DNA helicase VIII antibody
    • G3BP antibody
    • G3BP-1 antibody
    • G3bp1 antibody
    • G3BP1_HUMAN antibody
    • GAP binding protein antibody
    • GAP SH3 domain binding protein 1 antibody
    • GAP SH3 domain-binding protein 1 antibody
    • GTPase activating protein (SH3 domain) binding protein 1 antibody
    • hDH VIII antibody
    • Human DNA helicase VIII antibody
    • MGC111040 antibody
    • Ras GTPase activating protein binding protein 1 antibody
    • Ras GTPase activating protein SH3 domain binding protein antibody
    • Ras GTPase-activating protein-binding protein 1 antibody
    • RasGAP associated endoribonuclease G3BP antibody
    see all

Anti-G3BP antibody 图像

  • ab56574 staining G3BP in Human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with Triton X-100 and blocked with 5% BSA for 12 hours at 4°C. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 37°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.

    Top row - untreated cells. Bottom row - cells treated with sodium arsenite. Left - G3BP, Middle - Nucleus, Right - Merge.

    Stress granules are visible in cells treated with sodium arsenite, whereas G3BP is dispersed in the cytoplasm in untreated cells.

    See Abreview

  • G3BP antibody (ab56574) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human lymphoma.


  • Predicted band size : 52 kDa
    G3BP antibody (ab56574) at 1ug/lane + A-431 cell lysate at 25ug/lane.
  • ICC/IF image of ab56574 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56574, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HeLa cells stained with ab56574 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56574, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Anti-G3BP antibody (ab56574)参考文献

This product has been referenced in:
  • Simeoni F  et al. Proteomic analysis of the SIRT6 interactome: novel links to genome maintenance and cellular stress signaling. Sci Rep 3:3085 (2013). ICC/IF ; Human . Read more (PubMed: 24169447) »
  • Gao X  et al. Tudor-SN interacts with and co-localizes with G3BP in stress granules under stress conditions. FEBS Lett 584:3525-32 (2010). WB ; Human . Read more (PubMed: 20643132) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 60 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (epithelial)
Specification epithelial
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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提交于 Sep 16 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
Sample Human Cell (epithelial cells)
Specification epithelial cells
Permeabilization Yes - Triton x100
Fixative Paraformaldehyde
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提交于 Aug 25 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Sample Human Cell (Hela Cells)
Specification Hela Cells
Permeabilization Yes - Triton x100
Fixative Formaldehyde
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提交于 Aug 23 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Hepatocytes)
Specification Hepatocytes
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: RT°C
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提交于 Oct 04 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"