Anti-G-protein coupled receptor 30抗体(ab39742)
- 数据表
- 具体的参考文献 (15)
- 实验方案
概述
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产品名称Anti-G-protein coupled receptor 30抗体
参阅全部 G-protein coupled receptor 30 一抗 -
描述兔多克隆抗体to G-protein coupled receptor 30
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宿主Rabbit
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经测试应用适用于: IHC-Fr, WB, ICC/IF, IHC-Pmore details
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种属反应性与反应: Mouse, Rat, Human
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human GPCR GPR30.
(Peptide available as ab41565.)
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阳性对照
- ab39742 gives a positive signal in Human Brain Membrane Lysate, Human Brain Tissue Lysate (data not shown), and MCF7 Whole Cell Lysate (data not shown) This antibody gave a positive signal in the following cell types: U-87 MG.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Immunohistochemistry kits
应用
Our Abpromise guarantee covers the use of ab39742 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr | 1/200 - 1/400. | |
| WB | 1/250. Detects a band of approximately 55 kDa (predicted molecular weight: 42 kDa). | |
| ICC/IF | Use a concentration of 5 µg/ml. | |
| IHC-P | Use at an assay dependent concentration. PubMed: 24421881 |
靶标
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功能Receptor for estrogen.
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组织特异性Ubiquitously expressed, but is most abundant in placenta. In brain regions, expressed as a 2.8 kb transcript in basal forebrain, frontal cortex, thalamus, hippocampus, caudate and putamen.
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序列相似性Belongs to the G-protein coupled receptor 1 family.
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细胞定位Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Protein has been detected in the cell membrane, endoplasmic reticulum and Golgi apparatus. It is currently unclear whether this is a cell surface or intracellular receptor.
- Information by UniProt
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数据库链接
- Entrez Gene: 2852 Human
- Entrez Gene: 76854 Mouse
- Entrez Gene: 171104 Rat
- Omim: 601805 Human
- SwissProt: Q99527 Human
- SwissProt: Q8BMP4 Mouse
- SwissProt: O08878 Rat
- Unigene: 20961 Human
see all -
别名
- CEPR antibody
- Chemoattractant receptor-like 2 antibody
- Chemokine receptor-like 2 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-G-protein coupled receptor 30 antibody (ab39742)This image is courtesy of an Abreview submitted by Ruma Raha-Chowdhuryab39742 staining cultured rat primary astrocytes by ICC/IF. The cultured cells were fixed with 4% paraformaldehyde for 5 minutes and blocked with 10% donkey serum in 0.1% PBS-0.3% TritonX for 30 minutes at 24°C. The cultured cells were then stained with ab39742 at 1/500 in 0.3% TritonX with 0.1% PBS and 10% donkey serum for 4h at 24°C. An Alexa Fluro 488 donkey anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei were stained with 1.43µM Hoechst and can be observed in blue. In red astrocytes can be observed (monoclonal anti GFAP).
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Immunocytochemistry/ Immunofluorescence - Anti-G-protein coupled receptor 30 antibody (ab39742)ICC/IF image of ab39742 stained U87-MG cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab39742 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-G-protein coupled receptor 30 antibody (ab39742)Anti-G-protein coupled receptor 30 antibody (ab39742) at 1 µg/ml + Human brain normal tissue lysate - membrane extract (ab29456) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 55 kDa (why is the actual band size different from the predicted?)
GPR30 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-protein coupled receptor 30 antibody (ab39742)Image from Samartzis N et al., Reprod Biol Endocrinol. 2012 Apr 20;10:30. doi: 10.1186/1477-7827-10-30. Fig 3.; 20 April 2012, Reproductive Biology and Endocrinology 2012, 10:30Immunohistochemical analysis of Human ovarian endometriosis tissue, staining G-protein coupled receptor 30 with ab39742 at 1/50 dilution.
实验方案
文献
This product has been referenced in:
- Yan L et al. Effects of Ovariectomy in an hSOD1-G93A Transgenic Mouse Model of Amyotrophic Lateral Sclerosis (ALS). Med Sci Monit 24:678-686 (2018). Read more (PubMed: 29394243) »
- Li JJ et al. Expression Pattern of G-Protein-Coupled Estrogen Receptor in Myometrium of Uteri with and without Adenomyosis. Biomed Res Int 2017:5974693 (2017). Read more (PubMed: 29109960) »
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