Anti-GNAS抗体(ab235959)
Key features and details
- Rabbit polyclonal to GNAS
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-GNAS抗体 -
描述
兔多克隆抗体to GNAS -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human GNAS aa 300-450.
Database link: P84996 -
阳性对照
- WB: MCF7 and HepG2 whole cell lysate. IHC-P: Human brain and small intestine tissue. ICC/IF: MCF7 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purity >95% -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab235959于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/500 - 1/1000.
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WB |
1/500 - 1/5000.
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ICC/IF |
1/50 - 1/200.
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说明 |
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IHC-P
1/500 - 1/1000. |
WB
1/500 - 1/5000. |
ICC/IF
1/50 - 1/200. |
靶标
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功能
May inhibit the adenylyl cyclase-stimulating activity of guanine nucleotide-binding protein G(s) subunit alpha which is produced from the same locus in a different open reading frame. -
疾病相关
GNAS hyperfunction
ACTH-independent macronodular adrenal hyperplasia 1
Pseudohypoparathyroidism 1B
Colorectal cancer -
序列相似性
Belongs to the ALEX family. -
细胞定位
Cell membrane. Cell projection, ruffle. Predominantly associated with cell membrane ruffles. - Information by UniProt
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数据库链接
- Entrez Gene: 2778 Human
- SwissProt: P84996 Human
- Unigene: 125898 Human
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别名
- ALEX_HUMAN antibody
- Alternative gene product encoded by XL-exon antibody
- GNAS antibody
- Protein ALEX antibody
图片
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Paraffin-embedded human small intestine tissue stained for G protein alpha S using ab235959 at 1/600 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
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4% paraformaldehyde-fixed, 0.2% Triton X-100-permeabilized MCF7 (Human breast adenocarcinoma cell line) cells stained for G protein alpha S (Green) using ab235959 at 1/200 dilution in ICC/IF. Counterstained with DAPI (blue). The secondary antibody was Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).
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All lanes : Anti-GNAS antibody (ab235959) at 1/500 dilution
All lanes : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution -
Paraffin-embedded human brain tissue stained for G protein alpha S using ab235959 at 1/600 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab235959 尚未被引用在任何文献中。