Anti-G protein alpha Inhibitor 2抗体(ab20392)

概述

  • 产品名称Anti-G protein alpha Inhibitor 2抗体
    参阅全部 G protein alpha Inhibitor 2 一抗
  • 描述
    兔多克隆抗体to G protein alpha Inhibitor 2
  • 经测试应用适用于: WB, IP, ICC/IFmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Other Immunogen Type corresponding to Human G protein alpha Inhibitor 2.

性能

应用

Our Abpromise guarantee covers the use of ab20392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 40 kDa.
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

靶标

  • 功能Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division.
  • 序列相似性Belongs to the G-alpha family. G(i/o/t/z) subfamily.
  • 细胞定位Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cell membrane. Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Adenylate cyclase inhibiting G alpha protein antibody
    • Adenylate cyclase-inhibiting G alpha protein antibody
    • GIP antibody
    • GNAI 2 antibody
    • Gnai2 antibody
    • GNAI2_HUMAN antibody
    • GNAI2B antibody
    • GTP binding regulatory protein Gi alpha 2 chain antibody
    • Guanine nucleotide binding protein G protein alpha inhibiting activity polypeptide 2 antibody
    • Guanine nucleotide-binding protein G(i) subunit alpha-2 antibody
    • H LUCA15.1 antibody
    • H LUCA16.1 antibody
    • WUGSC:H LUCA15.1 antibody
    • WUGSC:H LUCA16.1 antibody
    see all

Anti-G protein alpha Inhibitor 2 antibody 图像

  • Lanes 1 & 3 : Anti-G protein alpha Inhibitor 2 antibody (ab20392) at 1/1000 dilution
    Lane 2 : No primary

    Lane 1 : Rat RH7777 whole cell lysate
    Lane 2 : Rat RH7777 whole cell lysate
    Lane 3 : Rat RH7777 whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    Lane 1 : No secondary
    Lanes 2 - 3 : Alkaline phosphatase conjugated goat anti-rabbit


    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 40 kDa


    Exposure time : 10 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

Anti-G protein alpha Inhibitor 2 antibody (ab20392)参考文献

This product has been referenced in:
  • Ramírez VT  et al. Wnt-5a/Frizzled9 Receptor Signaling through the Gao-Gß? Complex Regulates Dendritic Spine Formation. J Biol Chem 291:19092-107 (2016). Read more (PubMed: 27402827) »
  • Hassan B  et al. Alteration of the mu opioid receptor: Ca2+ channel signaling pathway in a subset of rat sensory neurons following chronic femoral artery occlusion. J Neurophysiol 112:3104-15 (2014). Read more (PubMed: 25231620) »

See all 4 Publications for this product

Product Wall

Thank you for sending the image again.

As my colleague mentioned you may need to increase the denaturing and reducing conditions with this specific cell line.

My suggestion is to lysate the cells with RIPA buffer, a recipe for this ...

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Thank you for your inquiry.

I am sorry to hear that you are getting results that are hard to interpret.

I believe the 120kDa band is not a complex containing G protein alpha Inhibitor 2 since you are using denaturing and reducing co...

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Thank you for contacting us.

The information I was able to obtain regarding the immunogen is as follows. Any further details are considered proprietary.

The immunogen for this antibody is a synthetic peptide surrounding amino acid ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (RH7777 Cells)
Loading amount 15 µg
Specification RH7777 Cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
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提交于 Jul 31 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"