Anti-G Protein alpha Inhibitor 1+2抗体(ab3522)

概述

  • 产品名称
    Anti-G Protein alpha Inhibitor 1+2抗体
  • 描述
    兔多克隆抗体to G Protein alpha Inhibitor 1+2
  • 经测试应用
    适用于: WB, IHC-P, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken, Guinea pig, Hamster, Cow, Dog, Xenopus laevis, Starfish
  • 免疫原

    Synthetic peptide corresponding to Human G Protein alpha Inhibitor 1+2 aa 346-355.
    Sequence:

    KNNLKDCGLF


    (Peptide available as ab5006)

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • Primary antibody说明
    The G protein family of signal transducers includes the alpha, beta, gamma subunits and the effector which are most clearly distinguished by their different alpha chains. The family also includes Gs and Gi, the stimulatory and inhibitory GTP-binding regulators of adenylate cyclase; Go, a protein of unknown function that is abundantly expressed in brain; and transducin 1 and 2, proteins involved in retinal phototransduction. The G proteins possess GTPase activity and are components of a complex membrane signaling system that includes membrane-bound receptors. The system exists to modulate extracellular signals as they are transmitted into the cell.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa.Can be blocked with G Protein alpha Inhibitor 1+2 peptide (ab5006).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use a concentration of 1 µg/ml.

靶标

图片

  • IHC image of ab3522 staining in Human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3522, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

文献

This product has been referenced in:
  • Doi M  et al. Gpr176 is a Gz-linked orphan G-protein-coupled receptor that sets the pace of circadian behaviour. Nat Commun 7:10583 (2016). WB . Read more (PubMed: 26882873) »
  • Ufer C & Germack R Cross-regulation between beta1- and beta3-adrenoceptors following chronic beta-adrenergic stimulation in neonatal rat cardiomyocytes. Br J Pharmacol 158:300-13 (2009). WB ; Rat . Read more (PubMed: 19719783) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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