- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
-
产品名称Anti-G-CSF抗体
参阅全部 G-CSF 一抗 -
描述兔多克隆抗体to G-CSF
-
宿主Rabbit
-
经测试应用适用于: IHC-Pmore details
-
种属反应性与反应: Human
预测可用于: Sheep, Horse, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan
-
免疫原
Synthetic peptide corresponding to Human G-CSF aa 1-100 conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: P09919 -
阳性对照
- IHC-P: Human lung FFPE tissue sections.
性能
-
形式Liquid
-
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
-
存储溶液pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度Immunogen affinity purified
-
克隆多克隆
-
同种型IgG
-
研究领域
相关产品
-
Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab112112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
-
功能Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
-
序列相似性Belongs to the IL-6 superfamily.
-
翻译后修饰O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).
-
细胞定位Secreted.
- Information by UniProt
-
数据库链接
- Entrez Gene: 1440 Human
- Omim: 138970 Human
- SwissProt: P09919 Human
- SwissProt: O02837 Pig
- SwissProt: Q28746 Sheep
- Unigene: 2233 Human
-
别名
- C17orf33 antibody
- Colony stimulating factor 3 (granulocyte) antibody
- Colony stimulating factor 3 antibody
see all
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-CSF antibody (ab112112)IHC image of G-CSF staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab112112, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
文献
This product has been referenced in:
- Liu Z et al. Prognostic value of granulocyte colony-stimulating factor in patients with non-metastatic clear cell renal cell carcinoma. Oncotarget 8:69961-69971 (2017). Read more (PubMed: 29050255) »
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"