Anti-G-CSF抗体[7E4F7] (ab204989)
Key features and details
- Mouse monoclonal [7E4F7] to G-CSF
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG2a
概述
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产品名称
Anti-G-CSF抗体[7E4F7]
参阅全部 G-CSF 一抗 -
描述
小鼠单克隆抗体[7E4F7] to G-CSF -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human G-CSF aa 1 to the C-terminus. (Purified from E.coli).
Database link: P09919 -
阳性对照
- Human G-CSF (aa1-207) recombinant protein; G-CSF (aa1-207)-hIgGFc transfected HEK293 cell lysate; Human esophageal cancer tissue; HeLa and A549 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purified from tissue culture supernatant. -
克隆
单克隆 -
克隆编号
7E4F7 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab204989于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/2000. Predicted molecular weight: 22 kDa.
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ICC/IF |
1/200 - 1/1000.
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IHC-P |
1/200 - 1/1000.
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说明 |
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WB
1/500 - 1/2000. Predicted molecular weight: 22 kDa. |
ICC/IF
1/200 - 1/1000. |
IHC-P
1/200 - 1/1000. |
靶标
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功能
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. -
序列相似性
Belongs to the IL-6 superfamily. -
翻译后修饰
O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells). -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 1440 Human
- Omim: 138970 Human
- SwissProt: P09919 Human
- Unigene: 2233 Human
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别名
- C17orf33 antibody
- Colony stimulating factor 3 (granulocyte) antibody
- Colony stimulating factor 3 antibody
see all
图片
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Anti-G-CSF antibody [7E4F7] (ab204989) at 1/500 dilution + Human G-CSF (aa1-207) recombinant protein
Predicted band size: 22 kDaExpected MWt is 48.2 kDa.
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All lanes : Anti-G-CSF antibody [7E4F7] (ab204989) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : G-CSF (aa1-207)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 22 kDa -
Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissue labeling G-CSF with ab204989 at 1/200 dilution with DAB staining.
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Immunofluorescent analysis of A549 cells labeling G-CSF with ab204989 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
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Immunofluorescent analysis of HeLa cells labeling G-CSF with ab204989 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
实验方案
数据表及文件
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Datasheet download
文献 (1)
ab204989 被引用在 1 文献中.
- Zhang JY et al. Vitamin D Promotes Trophoblast Cell Induced Separation of Vascular Smooth Muscle Cells in Vascular Remodeling via Induction of G-CSF. Front Cell Dev Biol 8:601043 (2020). PubMed: 33415106