概述

  • 产品名称Anti-FZR1抗体[AR38.2]
    参阅全部 FZR1 一抗
  • 描述
    小鼠单克隆抗体[AR38.2] to FZR1
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant full length protein

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • 纯度IgG fraction
  • 克隆单克隆
  • 克隆编号AR38.2
  • 骨髓瘤Sp2/0-Ag14
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab77885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2.5 µg/ml. Predicted molecular weight: 55 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 功能Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis.
  • 组织特异性Isoform 2 is expressed at high levels in heart, liver, spleen and some cancer cell lines whereas isoform 3 is expressed only at low levels in these tissues.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Belongs to the WD repeat CDC20/Fizzy family.
    Contains 7 WD repeats.
  • 翻译后修饰Phosphorylated during mitosis, probably by maturation promoting factor (MPF), leading to its dissociation of the APC/C. Following DNA damage, it is dephosphorylated by CDC14B in G2 phase, leading to its reassociation with the APC/C, and allowing an efficient G2 DNA damage checkpoint.
  • 细胞定位Cytoplasm and Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CDC20 like 1b antibody
    • CDC20 like protein 1 antibody
    • CDC20-like protein 1 antibody
    • CDC20C antibody
    • CDH1 antibody
    • Cdh1/Hct1 homolog antibody
    • Fizzy related protein 1 antibody
    • Fizzy related protein antibody
    • Fizzy related protein homolog antibody
    • Fizzy-related protein homolog antibody
    • Fizzy/cell division cycle 20 related 1 antibody
    • FYR antibody
    • FZR 1 antibody
    • FZR 2 antibody
    • Fzr antibody
    • FZR_HUMAN antibody
    • Fzr1 antibody
    • Fzr1 protein antibody
    • FZR2 antibody
    • HCDH 1 antibody
    • HCDH antibody
    • hCDH1 antibody
    • KIAA1242 antibody
    see all

Anti-FZR1 antibody [AR38.2] 图像

  • All lanes : Anti-FZR1 antibody [AR38.2] (ab77885) at 2.5 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : Murine myeloid cells


    Predicted band size : 55 kDa

Anti-FZR1 antibody [AR38.2] (ab77885)参考文献

This product has been referenced in:
  • Holt JE  et al. Spatial regulation of APCCdh1-induced cyclin B1 degradation maintains G2 arrest in mouse oocytes. Development 137:1297-304 (2010). WB ; Mouse . Read more (PubMed: 20223764) »

See 1 Publication for this product

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