概述

  • 产品名称Anti-FZR1抗体
    参阅全部 FZR1 一抗
  • 描述
    小鼠单克隆抗体to FZR1
  • 经测试应用适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: MDQDYERRLL RQIVIQNENT MPRVTEMRRT LTPASSPVSS PSKHGDRFIP SRAGANWSVN FHRINENEKS PSQNRKAKDA TSDNGKDGLA YSALLKNELL G, corresponding to amino acids 1-102 of human FZR1 (NP_057347.2), with a 26 kDa proprietary tag.

  • 阳性对照
    • HeLa cell lysate This antibody gave a positive result when used in the following methanol fixed cell lines: HepG2, MCF-7.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab89535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 55 kDa.
ELISA Use at an assay dependent concentration.

靶标

  • 功能Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis.
  • 组织特异性Isoform 2 is expressed at high levels in heart, liver, spleen and some cancer cell lines whereas isoform 3 is expressed only at low levels in these tissues.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Belongs to the WD repeat CDC20/Fizzy family.
    Contains 7 WD repeats.
  • 翻译后修饰Phosphorylated during mitosis, probably by maturation promoting factor (MPF), leading to its dissociation of the APC/C. Following DNA damage, it is dephosphorylated by CDC14B in G2 phase, leading to its reassociation with the APC/C, and allowing an efficient G2 DNA damage checkpoint.
  • 细胞定位Cytoplasm and Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CDC20 like 1b antibody
    • CDC20 like protein 1 antibody
    • CDC20-like protein 1 antibody
    • CDC20C antibody
    • CDH1 antibody
    • Cdh1/Hct1 homolog antibody
    • Fizzy related protein 1 antibody
    • Fizzy related protein antibody
    • Fizzy related protein homolog antibody
    • Fizzy-related protein homolog antibody
    • Fizzy/cell division cycle 20 related 1 antibody
    • FYR antibody
    • FZR 1 antibody
    • FZR 2 antibody
    • Fzr antibody
    • FZR_HUMAN antibody
    • Fzr1 antibody
    • Fzr1 protein antibody
    • FZR2 antibody
    • HCDH 1 antibody
    • HCDH antibody
    • hCDH1 antibody
    • KIAA1242 antibody
    see all

Anti-FZR1 antibody 图像

  • ICC/IF image of ab89535 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab89535 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in methanol fixed (100%, 5min) MCF-7 cells at 10ug/ml.

  • Anti-FZR1 antibody (ab89535) at 5 µg/ml + immunogen at 0.2 µg

    Predicted band size : 55 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
  • Anti-FZR1 antibody (ab89535) at 5 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size : 55 kDa
    Observed band size : 55 kDa

Anti-FZR1 antibody (ab89535)参考文献

This product has been referenced in:
  • Lu H  et al. The E3 ligase APC/C-Cdh1 regulates MEF2A-dependent transcription by targeting SUMO-specific protease 2 for ubiquitination and degradation. Cell Cycle 13:3892-902 (2014). WB . Read more (PubMed: 25483061) »

See 1 Publication for this product

Product Wall

The Uniprot link on the datasheet http://www.uniprot.org/uniprot/Q9UM11 is showing FZR1. I understand that this is not enough. I apologize for the misleading information. We are looking into changing the name of the product. I have asked our a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"