Anti-FZD3抗体(ab102965)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to FZD3
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Chicken, Cow, Dog, Pig, Macaque monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human FZD3.

  • 阳性对照
    • This antibody gave a positive signal in Human skeletal muscle tissue lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab102965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 76 kDa).

靶标

  • 功能
    Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
  • 组织特异性
    Widely expressed. Relatively high expression in the CNS, including regions of the limbic system, in kidney, pancreas, skeletal muscle, uterus and testis.
  • 序列相似性
    Belongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • 结构域
    Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
    The FZ domain is involved in binding with Wnt ligands.
  • 细胞定位
    Membrane. Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Frizzled 3 antibody
    • Frizzled 3 seven transmembrane spanning receptor antibody
    • Frizzled family receptor 3 antibody
    • Frizzled homolog 3 (Drosophila) antibody
    • Frizzled homolog 3 antibody
    • Frizzled-3 antibody
    • Frizzled3 antibody
    • Fz 3 antibody
    • Fz-3 antibody
    • Fz3 antibody
    • FZD 3 antibody
    • Fzd3 antibody
    • FZD3_HUMAN antibody
    • hFz 3 antibody
    • hFz3 antibody
    see all

Anti-FZD3 antibody 图像

  • Anti-FZD3 antibody (ab102965) at 1 µg/ml + Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 76 kDa
    Observed band size : 74 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 34 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

实验方案

Anti-FZD3 antibody (ab102965)参考文献

ab102965 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (E9.5 mouse embryo section)
Specification
E9.5 mouse embryo section
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Fixative
Paraformaldehyde
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提交于 Jan 28 2015

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