Anti-Fyn抗体[3G11-F9] (ab76934)
Key features and details
- Mouse monoclonal [3G11-F9] to Fyn
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Fyn抗体[3G11-F9]
参阅全部 Fyn 一抗 -
描述
小鼠单克隆抗体[3G11-F9] to Fyn -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein, corresponding to amino acids 1-483 of Human Fyn (AAH32496), with a 26kDa enzyme tag.
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阳性对照
- FYN transfected 293T cell lysate
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常规说明
This product was changed from ascites to tissue culture supernatant on 30th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.4
Constituent: PBS -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
3G11-F9 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76934于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
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说明 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). |
靶标
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功能
Tyrosine-protein kinase implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels, with isoform 2 showing the greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. Blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC. Phosphorylates RUNX3. -
组织特异性
Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T lymphocytes. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
细胞定位
Cell membrane. Present and active in lipid rafts. Present in cell body and along the process of mature and developing oligodendroyctes. - Information by UniProt
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数据库链接
- Entrez Gene: 2534 Human
- Omim: 137025 Human
- SwissProt: P06241 Human
- Unigene: 390567 Human
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形式
This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request. -
别名
- C syn protooncogene antibody
- Fyn antibody
- FYN oncogene related to SRC FGR YES antibody
see all
图片
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Anti-Fyn antibody [3G11-F9] (ab76934) at 5 µg/ml + Immunogen at 0.2 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size: 60 kDa
Observed band size: 86 kDa why is the actual band size different from the predicted?This image was generated using the ascites version of the product.
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All lanes : Anti-Fyn antibody [3G11-F9] (ab76934) at 5 µg/ml
Lane 1 : Transfected 293T cell lysate
Lane 2 : Untransfected 293T cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDaThis image was generated using the ascites version of the product.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab76934 尚未被引用在任何文献中。