RabMAb

Anti-FYB抗体[EP2546Y] (ab76103)

概述

  • 产品名称Anti-FYB抗体[EP2546Y]
    参阅全部 FYB 一抗
  • 描述
    兔单克隆抗体[EP2546Y] to FYB
  • 经测试应用适用于: ICC/IF, WB, IHC-P, Flow Cytmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human FYB aa 50-150 (N terminal). The exact sequence is proprietary.
    Database link: O15117

  • 阳性对照
    • TF-1 lysate. Human tonsil tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Alternative versions available:

    Anti-FYB antibody (Alexa Fluor® 488) [EP2546Y] (ab206707)

性能

应用

Our Abpromise guarantee covers the use of ab76103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/250.
WB 1/5000 - 1/10000. Predicted molecular weight: 85 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Acts as an adapter protein of the FYN and LCP2 signaling cascades in T cells. Modulates the expression of interleukin-2 (IL-2). Involved in platelet activation. Prevents the degradation of SKAP1 and SKAP2.
    • 组织特异性Expressed in hematopoietic tissues such as myeloid and T-cells, spleen and thymus. Not expressed in B-cells, nor in non-lymphoid tissues.
    • 序列相似性Contains 1 SH3 domain.
    • 翻译后修饰T-cell receptor ligation leads to increased tyrosine phosphorylation.
    • 细胞定位Cytoplasm. Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ADAP antibody
      • Adhesion and degranulation promoting adaptor protein antibody
      • FYB 120/130 antibody
      • Fyb antibody
      • FYB-120/130 antibody
      • FYB_HUMAN antibody
      • FYN binding protein antibody
      • FYN T binding protein antibody
      • FYN-binding protein antibody
      • FYN-T-binding protein antibody
      • p120/p130 antibody
      • PRO0823 antibody
      • SLAP 130 antibody
      • SLAP-130 antibody
      • SLAP130 antibody
      • SLP 76 associated phosphoprotein antibody
      • SLP-76-associated phosphoprotein antibody
      • SLP76 associated phosphoprotein antibody
      see all

    Anti-FYB antibody [EP2546Y] 图像

    • Anti-FYB antibody [EP2546Y] (ab76103) at 1/10000 dilution + TF-1 lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 85 kDa
      Observed band size : 110 kDa (why is the actual band size different from the predicted?)
    • ab76103, at 1/250 dilution, staining FYB in paraffin-embedded human tonsil tissue by Immunohistochemistry.
    • ICC/If image of CHO cell expressing human ADAP using ab 76103. The cells were fixed in formaldehyde and permeabilised using 0.2% Triton X100 for 5 mins. The cells were them blocked using 10% serum for 1 hour.

      See Abreview

    Anti-FYB antibody [EP2546Y] (ab76103)参考文献

    ab76103 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (CHO cell expressing human ADAP)
    Specification CHO cell expressing human ADAP
    Fixative Formaldehyde
    Permeabilization Yes - 0.2% Triton X100, 5 min
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 Jan 04 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (transfected CHO cells)
    Loading amount 40 µg
    Specification transfected CHO cells
    Gel Running Conditions Reduced Denaturing (7.5%)
    Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 Sep 08 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"