重组Anti-FYB抗体[EP2546Y] (ab76103)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2546Y] to FYB
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-FYB抗体[EP2546Y]
参阅全部 FYB 一抗 -
描述
兔单克隆抗体[EP2546Y] to FYB -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-Pmore details
不适用于: IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human FYB aa 50-150 (N terminal). The exact sequence is proprietary.
Database link: O15117 -
阳性对照
- WB: TF-1 lysate. IHC-P: Human tonsil tissue. Flow Cyt (intra): TF-1 cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP2546Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76103于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB | (1) |
1/5000 - 1/10000. Predicted molecular weight: 85 kDa.
|
IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
说明 |
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Flow Cyt (Intra)
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/5000 - 1/10000. Predicted molecular weight: 85 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Acts as an adapter protein of the FYN and LCP2 signaling cascades in T cells. Modulates the expression of interleukin-2 (IL-2). Involved in platelet activation. Prevents the degradation of SKAP1 and SKAP2. -
组织特异性
Expressed in hematopoietic tissues such as myeloid and T-cells, spleen and thymus. Not expressed in B-cells, nor in non-lymphoid tissues. -
序列相似性
Contains 1 SH3 domain. -
翻译后修饰
T-cell receptor ligation leads to increased tyrosine phosphorylation. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 2533 Human
- Omim: 602731 Human
- SwissProt: O15117 Human
- Unigene: 370503 Human
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别名
- ADAP antibody
- Adhesion and degranulation promoting adaptor protein antibody
- FYB 120/130 antibody
see all
图片
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Anti-FYB antibody [EP2546Y] (ab76103) at 1/10000 dilution + TF-1 cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 85 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted? -
ab76103, at 1/250 dilution, staining FYB in paraffin-embedded human tonsil tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Intracellular Flow Cytometry analysis of TF-1 (Human Erythroleukemia erythroblast) cells labeling FYB with purified ab76103 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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