概述

  • 产品名称Anti-FXYD1抗体
    参阅全部 FXYD1 一抗
  • 描述
    兔多克隆抗体to FXYD1
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow, Dog, Macaque Monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human FXYD1.

    (Peptide available as ab88161.)

  • 阳性对照
    • This antibody gave a positive signal in the following tissue lysates: Human Brain; Human Brain Membrane; Human Heart; Human Skeletal Muscle

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab76597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa).
IHC-P Use at an assay dependent concentration.

靶标

  • 相关性PLM (FXYD1)is a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. PLM may be phosphorylated by several kinases, including protein kinase A, protein kinase C, NIMA kinase, and myotonic dystrophy kinase. It is thought to form an ion channel or regulate ion channel activity.
  • 细胞定位Membrane; single-pass type I membrane protein
  • 数据库链接
  • 别名
    • FXYD domain containing ion transport regulator 1 antibody
    • Phospholemman antibody
    • PLM antibody

Anti-FXYD1 antibody 图像

  • All lanes : Anti-FXYD1 antibody (ab76597) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human brain normal tissue lysate - membrane extract (ab29456)
    Lane 3 : Human heart tissue lysate - total protein (ab29431)
    Lane 4 : Human skeletal muscle tissue lysate - total protein (ab29330)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 10 kDa
    Observed band size : 10 kDa


    Exposure time : 5 minutes
  • ab76597 at a 1/100 dilution, staining mouse FXYD1 in heart and muscle tissue by Immunohistochemistry, Formalin fixed Paraffin embedded tissue.

    See Abreview

Anti-FXYD1 antibody (ab76597)参考文献

This product has been referenced in:
  • Howie J  et al. Substrate recognition by the cell surface palmitoyl transferase DHHC5. Proc Natl Acad Sci U S A 111:17534-9 (2014). Read more (PubMed: 25422474) »
  • Matagne V  et al. Correcting deregulated Fxyd1 expression ameliorates a behavioral impairment in a mouse model of Rett syndrome. Brain Res 1496:104-14 (2013). WB ; Mouse . Read more (PubMed: 23246925) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - other (Human Cardiomyocytes)
Loading amount 20 µg
Specification Human Cardiomyocytes
Gel Running Conditions Reduced Denaturing (8% SDS-PAGE gel with a 5% stacking gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Prof. Paul Townsend

Verified customer

提交于 Apr 27 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Mouse cardiomyocyte)
Loading amount 20 µg
Specification Mouse cardiomyocyte
Gel Running Conditions Reduced Denaturing (8% SDS-PAGE gel with a 5% stacking gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Prof. Paul Townsend

Verified customer

提交于 Apr 27 2010

Application Western blot
Sample Rat Tissue lysate - whole (Rat cardiomyocyte)
Loading amount 20 µg
Specification Rat cardiomyocyte
Gel Running Conditions Reduced Denaturing (8% SDS-PAGE gel with a 5% stacking gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Prof. Paul Townsend

Verified customer

提交于 Apr 20 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (heart, muscle)
Specification heart, muscle
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Mar 25 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Brain, Intestine)
Loading amount 20 µg
Specification Brain, Intestine
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Mar 04 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"