概述

  • 产品名称Anti-FXR1抗体
    参阅全部 FXR1 一抗
  • 描述
    山羊多克隆抗体to FXR1
  • 特异性This antibody is expected to recognise all reported isoforms
  • 经测试应用适用于: IHC-P, ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Dog
  • 免疫原

    Synthetic peptide:

    RIEGDNENKLPRED

    , corresponding to amino acids 317/330 of Human FXR1

  • 阳性对照
    • NIH/3T3 cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab51970 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 69 kDa).
ELISA 1/128000.

靶标

Anti-FXR1 antibody 图像

  • Anti-FXR1 antibody (ab51970) at 0.2 µg/ml + NIH/3T3 cell lysate in RIPA buffer at 35 µg

    Predicted band size : 69 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab51970 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51970, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab51970 staining in human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab51970, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-FXR1 antibody (ab51970)参考文献

This product has been referenced in:
  • Oldenburg AR  et al. Deregulation of Fragile X-related protein 1 by the lipodystrophic lamin A p.R482W mutation elicits a myogenic gene expression program in preadipocytes. Hum Mol Genet N/A:N/A (2013). ICC/IF ; Human . Read more (PubMed: 24108105) »
  • Xu XL  et al. FXR1P But Not FMRP Regulates the Levels of Mammalian Brain-Specific microRNA-9 and microRNA-124. J Neurosci 31:13705-9 (2011). Read more (PubMed: 21957233) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (LNCaP prostate cancer cell line)
Loading amount 20000 cells
Specification LNCaP prostate cancer cell line
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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提交于 Oct 09 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"