Anti-FUZ抗体(ab122742)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to FUZ
  • 经测试应用
    适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: LLRNFYTLVT STHFPPEPGP PEKTEDEVYQ AQLPRACYLV LGTEEPGTGV RLVALQLGLR RLLLLLSPQS PTHGLRSLAT HTLHALTP, corresponding to amino acids 329-416 of Human FUZ.

  • 阳性对照
    • Human smooth muscle tissue

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab122742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 4 µg/ml.

靶标

  • 功能
    Probable planar cell polarity effector involved in cilium biogenesis. May regulate protein and membrane transport to the cilium. May regulate the morphogenesis of hair follicles which depends on functional primary cilia.
  • 序列相似性
    Belongs to the fuzzy family.
  • 细胞定位
    Cytoplasm. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ22688 antibody
    • fuz antibody
    • FUZZY_HUMAN antibody
    • Fy antibody
    • Protein fuzzy homolog antibody
    see all

图片

  • Immunocytochemistry/immunofluorescence analysis of HEK293 cells labelling FUZ with ab122742 shows positive staining in the cytoplasm.
  • ab122742 at 1/50 dilution staining FUZ in Paraffin-embedded Human smooth muscle tissue by Immunohistochemistry.
  • Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
    Lane 2: Negative control (vector only transfected HEK293T lysate)
    Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells. Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
    Lane 2: Negative control (vector only transfected HEK293T lysate)
    Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.

文献

ab122742 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for contacting Abcam.

Under our Abpromise we guarantee both ab122778 and ab122742 to work in IF on human samples. If you were to purchase either of them and they were not to perform as stated, then we would be happy to either replac...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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