概述

  • 产品名称Anti-FUT8抗体
    参阅全部 FUT8 一抗
  • 描述
    小鼠多克隆抗体to FUT8
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length human FUT8 protein (NP_835367.1)

  • 阳性对照
    • FUT8 transfected 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab76791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 67 kDa.

靶标

  • 功能Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans.
  • 通路Protein modification; protein glycosylation.
  • 序列相似性Belongs to the glycosyltransferase 23 family.
    Contains 1 SH3 domain.
  • 细胞定位Golgi apparatus > Golgi stack membrane. Membrane-bound form in trans cisternae of Golgi.
  • Information by UniProt
  • 数据库链接
  • 形式The expression of this gene may contribute to the malignancy of cancer cells and to their invasive and metastatic capabilities. There are 2 isoforms produced by alternative splicing. Immunogen is not present in isoform 2 (also known as Retinal).
  • 别名
    • 6-fucosyltransferase antibody
    • Alpha (1,6) fucosyltransferase antibody
    • Alpha-(1,6)-fucosyltransferase antibody
    • Alpha1 6FucT antibody
    • alpha1-6FucT antibody
    • Fucosyltransferase 8 (alpha (1,6) fucosyltransferase) antibody
    • Fucosyltransferase 8 antibody
    • FUT8 antibody
    • FUT8_HUMAN antibody
    • GDP fucose glycoprotein fucosyltransferase antibody
    • GDP L Fuc:N acetyl beta D glucosaminide alpha1,6 fucosyltransferase antibody
    • GDP-fucose--glycoprotein fucosyltransferase antibody
    • GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1 antibody
    • Glycoprotein 6 alpha L fucosyltransferase antibody
    • Glycoprotein 6-alpha-L-fucosyltransferase antibody
    • MGC26465 antibody
    see all

Anti-FUT8 antibody 图像

  • All lanes : Anti-FUT8 antibody (ab76791) at 5 µg/ml

    Lane 1 : FUC8 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 67 kDa
    Observed band size : 73 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab76791 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76791, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-FUT8 antibody (ab76791)参考文献

This product has been referenced in:
  • Wang M  et al. MiR-198 represses tumor growth and metastasis in colorectal cancer by targeting fucosyl transferase 8. Sci Rep 4:6145 (2014). Read more (PubMed: 25174450) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Melanoma)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 40 µg
Specification Melanoma
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Praveen Agrawal

Verified customer

提交于 Oct 19 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"