Anti-FUT6抗体(ab104270)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to FUT6
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 110-140 of Human FUT6

  • 阳性对照
    • HL-60 cell line lysates, bladder carcinoma tissue

性能

应用

Our Abpromise guarantee covers the use of ab104270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 42 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 相关性
    Enzyme involved in the biosynthesis of the E-Selectin ligand, sialyl-Lewis X. Catalyzes the transfer of fucose from GDP-beta-fucose to alpha-2,3 sialylated substrates.
  • 细胞定位
    Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note: Membrane-bound form in trans cisternae of Golgi.
  • 数据库链接
  • 别名
    • Alpha (1,3) fucosyltransferase antibody
    • EC=2.4.1.65 antibody
    • FCT3A antibody
    • FT1A antibody
    • Fuc TVI antibody
    • fucosyltransferase 6 (alpha (1,3) fucosyltransferase) antibody
    • Fucosyltransferase 6 antibody
    • Fucosyltransferase VI antibody
    • FucT VI antibody
    • Galactoside 3 L fucosyltransferase antibody
    see all

图片

  • Anti-FUT6 antibody (ab104270) at 1/100 dilution + HL-60 cell line lysates at 35 µg

    Predicted band size : 42 kDa
  • IHC analysis of FUT6 expression in formalin fixed and paraffin embedded bladder carcinoma using 1/50 ab104270 followed by peroxidase conjugation of the secondary antibody and DAB staining.

文献

ab104270 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Sample
Human Cell lysate - whole cell (breast cancer)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
100000 cells
Specification
breast cancer
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Nov 30 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册