antigen sequence, corresponding to C terminal amino acids 348-482 of Human FUT11.
- Human liver tissue; Transfected HEK293T Cell lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
Protein modification; protein glycosylation.
Belongs to the glycosyltransferase 10 family.
Golgi apparatus > Golgi stack membrane.
Information by UniProt
- 3)-fucosyltransferase 11 antibody
- Alpha (1,3) fucosyltransferase 11 antibody
- Alpha-(1 antibody
Anti-FUT11 antibody 图像
Immunocytochemistry/ Immunofluorescence - Anti-FUT11 antibody (ab121411)
Immunofluorescent staining of Human cell line A-431 shows positivity in nuclear membrane and golgi apparatus. Recommended concentration of ab121411 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUT11 antibody (ab121411)
ab121411 at 1/50 dilution staining FUT11 in paraffin-embedded Human liver tissue by Immunohistochemistry.
Western blot - Anti-FUT11 antibody (ab121411)
All lanes : Anti-FUT11 antibody (ab121411) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : FUT11-transfected HEK293T over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))
Developed using the ECL technique
Anti-FUT11 antibody (ab121411)参考文献
has not yet been referenced specifically in any publications.
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