Anti-Furin抗体[MM0298-4G31] (ab89558)

概述

  • 产品名称Anti-Furin抗体[MM0298-4G31]
    参阅全部 Furin 一抗
  • 描述
    小鼠单克隆抗体[MM0298-4G31] to Furin
  • 经测试应用适用于: Flow Cyt, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant Human Furin

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and filtered through a 0.2 µm filter.
  • 克隆单克隆
  • 克隆编号MM0298-4G31
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab89558 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB 1/500 - 1/1000. Predicted molecular weight: 88 kDa.
IHC-P 1/20 - 1/100.

靶标

  • 功能Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif.
  • 组织特异性Seems to be expressed ubiquitously.
  • 序列相似性Belongs to the peptidase S8 family. Furin subfamily.
    Contains 1 homo B/P domain.
  • 结构域Contains a cytoplasmic domain responsible for its TGN localization and recycling from the cell surface.
  • 翻译后修饰The inhibition peptide, which plays the role of an intramolecular chaperone, is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Following transport to the trans Golgi, a second cleavage within the inhibition propeptide results in propeptide dissociation and furin activation.
    Phosphorylation is required for TGN localization of the endoprotease. In vivo, exists as di-, mono- and non-phosphorylated forms.
  • 细胞定位Golgi apparatus > trans-Golgi network membrane. Cell membrane. Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Dibasic processing enzyme antibody
    • Dibasic-processing enzyme antibody
    • FES upstream region antibody
    • FUR antibody
    • FURIN antibody
    • Furin membrane associated receptor protein antibody
    • FURIN_HUMAN antibody
    • PACE antibody
    • Paired basic amino acid residue cleaving enzyme antibody
    • Paired basic amino acid residue-cleaving enzyme antibody
    • PCSK3 antibody
    • Proprotein convertase subtilisin/kexin type 3 antibody
    • SPC1 antibody
    see all

Anti-Furin antibody [MM0298-4G31] 图像

  • Overlay histogram showing HeLa cells stained with ab89558 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab89558, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-Furin antibody [MM0298-4G31] (ab89558)参考文献

ab89558 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Permeabilization Yes - 0.5% Triton X100 in PBS
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

提交于 Jan 28 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"