概述

  • 产品名称Anti-Furin抗体- Aminoterminal end
    参阅全部 Furin 一抗
  • 描述
    兔多克隆抗体to Furin - Aminoterminal end
  • 特异性ab28547 recognizes subtilisin-like serine protease furin.
  • 经测试应用适用于: WB, IHC-Frmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Human Furin (N terminal).
    (Peptide available as ab41397)

  • 阳性对照
    • Furin is ubiquitously expressed at low levels, but found in highest concentration in the liver and kidneys, lower levels in brain, spleen, thymus, and smaller levels in most other tissues. Endogenous furin was detected in lysates from LNCaP and DU145 prostate carcinoma cell lines.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.4
    Preservative: 0.05% Sodium azide
    Constituents: 50% Glycerol, 2.9% Sodium chloride, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This antibody has been peptide-affinity purified.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab28547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Predicted molecular weight: 88 kDa.

1/1,000 when using colorimetric substrates such as BCIP/NBT and 1/5,000 for chemiluminescent substrates. Glycosylation and other post-translational modifications make the pre-pro furin run at 110-104 kDa, the mature furin at 98-95 kDa and shed furin at 90 kDa. Dilution optimised using Chromogenic detection.

IHC-Fr Use at an assay dependent concentration. PubMed: 20454619

靶标

  • 功能Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif.
  • 组织特异性Seems to be expressed ubiquitously.
  • 序列相似性Belongs to the peptidase S8 family. Furin subfamily.
    Contains 1 homo B/P domain.
  • 结构域Contains a cytoplasmic domain responsible for its TGN localization and recycling from the cell surface.
  • 翻译后修饰The inhibition peptide, which plays the role of an intramolecular chaperone, is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Following transport to the trans Golgi, a second cleavage within the inhibition propeptide results in propeptide dissociation and furin activation.
    Phosphorylation is required for TGN localization of the endoprotease. In vivo, exists as di-, mono- and non-phosphorylated forms.
  • 细胞定位Golgi apparatus > trans-Golgi network membrane. Cell membrane. Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Dibasic processing enzyme antibody
    • Dibasic-processing enzyme antibody
    • FES upstream region antibody
    • FUR antibody
    • FURIN antibody
    • Furin membrane associated receptor protein antibody
    • FURIN_HUMAN antibody
    • PACE antibody
    • Paired basic amino acid residue cleaving enzyme antibody
    • Paired basic amino acid residue-cleaving enzyme antibody
    • PCSK3 antibody
    • Proprotein convertase subtilisin/kexin type 3 antibody
    • SPC1 antibody
    see all

Anti-Furin antibody - Aminoterminal end 图像

  • All lanes : Anti-Furin antibody - Aminoterminal end (ab28547) at 1/1000 dilution

    Lane 1 : Furin at 0.05 µg
    Lane 2 : Furin at 0.01 µg
    Lane 3 : Furin at 0.001 µg


    Predicted band size : 88 kDa
    Observed band size : 88 kDa
  • ab28547 staining Furin (red) in mouse endothelial tissue by Immunohistochemistry (Frozen sections).
    Mice were perfused, tumor and control organs were dissected and frozen in O.C.T. embedding medium. 10 µm cryosections were washed with PBS, fixed for 15 minutes in ice-cold methanol and air-dried. Primary antibody was used at a dilution of 1/1500. The secondary antibody was an Alexa Fluor 594-labeled IgG at a 1/300 dilution. Nuclei are visualized by DAPI staining (blue).

Anti-Furin antibody - Aminoterminal end (ab28547)参考文献

This product has been referenced in:
  • Sathyamurthy M  et al. Characterization and expression of proprotein convertases in CHO cells: Efficient proteolytic maturation of human bone morphogenetic protein-7. Biotechnol Bioeng N/A:N/A (2014). WB . Read more (PubMed: 25219685) »
  • Cekic M  et al. Progesterone treatment alters neurotrophin/proneurotrophin balance and receptor expression in rats with traumatic brain injury. Restor Neurol Neurosci 30:115-26 (2012). WB ; Rat . Read more (PubMed: 22232032) »

See all 3 Publications for this product

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