概述

  • 产品名称Anti-Furin抗体
    参阅全部 Furin 一抗
  • 描述
    兔多克隆抗体to Furin
  • 特异性Detects Furin from canine and mouse cells as well as transfected human Furin. This antibody does not detect endogenous Furin from BSC-40, HeLa, J774A.1 BPAEC, or CHO cells nor from rat skeletal muscle, spleen, kidney, ovary, testes, heart, or brain tissue.
  • 经测试应用适用于: ICC, Inhibition Assay, ICC/IF, IP, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Hamster, Dog, Human, Pig, Non Human Primates
  • 免疫原

    Synthetic peptide corresponding to Human Furin aa 780-793.
    Sequence:

    RGERTAFIKDQSAL


    (Peptide available as ab4989)

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • Primary antibody说明Furin is a membrane-associated, calcium-dependent, serine protease that belongs to the subtilisin-like prohormone convertase (PC) family. Members of this family of cellular enzymes cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. Furin and other PC family members share structural similarities which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. Furin is the only known PC to possess a transmembrane domain. Cleavage of target proteins occurs at the carboxyl-terminus of the furin consensus sequence, RX(K/R)R. It has been shown that the acidic peptide sequence, C771PSDSEEDEG780, localizes furin to the trans-Golgi-network. Phosphorylation of serine residues within this region modulates intracellular routing of Furin protein. An additional signaling domain includes the tetrapeptide sequence, Y759KGL762, which directs internalization from the cell surface.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent concentration.
Inhibition Assay Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 18174282
IP 1/100.
WB Use at an assay dependent concentration. Detects a band of approximately 100 kDa (predicted molecular weight: 87 kDa).Can be blocked with Furin peptide (ab4989).

Representing Furin in BSC-40 cells transfected with the human Furin gene.

IHC-P Use at an assay dependent concentration. PubMed: 23418414

靶标

  • 功能Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif.
  • 组织特异性Seems to be expressed ubiquitously.
  • 序列相似性Belongs to the peptidase S8 family. Furin subfamily.
    Contains 1 homo B/P domain.
  • 结构域Contains a cytoplasmic domain responsible for its TGN localization and recycling from the cell surface.
  • 翻译后修饰The inhibition peptide, which plays the role of an intramolecular chaperone, is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Following transport to the trans Golgi, a second cleavage within the inhibition propeptide results in propeptide dissociation and furin activation.
    Phosphorylation is required for TGN localization of the endoprotease. In vivo, exists as di-, mono- and non-phosphorylated forms.
  • 细胞定位Golgi apparatus > trans-Golgi network membrane. Cell membrane. Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Dibasic processing enzyme antibody
    • Dibasic-processing enzyme antibody
    • FES upstream region antibody
    • FUR antibody
    • FURIN antibody
    • Furin membrane associated receptor protein antibody
    • FURIN_HUMAN antibody
    • PACE antibody
    • Paired basic amino acid residue cleaving enzyme antibody
    • Paired basic amino acid residue-cleaving enzyme antibody
    • PCSK3 antibody
    • Proprotein convertase subtilisin/kexin type 3 antibody
    • SPC1 antibody
    see all

Anti-Furin antibody 图像

  • Immunolocalization of endogenous furin in mouse 3T3 cells with ab3467.

  • All lanes : Anti-Furin antibody (ab3467) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 87 kDa
    Observed band size : 63 kDa (why is the actual band size different from the predicted?)


    Exposure time : 90 seconds
    The band observed at 63 kDa could potentially be a cleaved form of Furin due to the presence of a 24 amino acid signal peptide and a 83 amino acid propeptide.
  • ab3467 staining Furin in human A549 cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed with paraformaldehyde, permeabilized using 0.1% Triton X-100, blocked with 1% BSA/ 5%FBS/PBS for 1 hour at 25°C and then incubated with ab3467 at a 1/100 dilution for 1 hour at 25°C. The secondary used was an Alexa-Fluor 546 conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.

    Cells were counterstained with DAPI (436nM, 5 mins) and Phalloidin-633 (1unit/coverslip) and mounted with prolong gold. No staining seen in secondary control and furin staining was punctate and throughout the cytoplasm.

    See Abreview

Anti-Furin antibody (ab3467)参考文献

This product has been referenced in:
  • Yang X  et al. Proprotein convertase furin regulates apoptosis and proliferation of granulosa cells in the rat ovary. PLoS One 8:e50479 (2013). IHC-P ; Rat . Read more (PubMed: 23418414) »
  • Valero-Muñoz M  et al. Relevance of vascular peroxisome proliferator-activated receptor ? coactivator-1a to molecular alterations in atherosclerosis. Exp Physiol 98:999-1008 (2013). Read more (PubMed: 23335007) »

See all 8 Publications for this product

Product Wall

Sorry for this misunderstanding.

We believe that the antibody recognizes the mature form of the protein. The pro-peptide is expected at 87kDa and once the signal and pro-peptide are cleaved off the protein, it migrates at 63 kDa.

I ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (3T3 fibroblasts)
Specification 3T3 fibroblasts
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% TX-100
Blocking step 1% BSA/ 5%FBS/PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 12 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (A549 lung epithelial cell line)
Specification A549 lung epithelial cell line
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% TX-100
Blocking step 1% BSA/ 5%FBS/PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 12 2012

Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. We have received a favourable review of our Furin Convertase antibody (ab3467) albeit with acknowledgement of the detection of some non-specif...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"