Anti-Fumarylacetoacetate hydrolase抗体(ab83770)

概述

  • 产品名称Anti-Fumarylacetoacetate hydrolase抗体
    参阅全部 Fumarylacetoacetate hydrolase 一抗
  • 描述
    兔多克隆抗体to Fumarylacetoacetate hydrolase
  • 经测试应用适用于: IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Guinea pig, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (SFIPVAEDSD FPIHNLPYGV FSTRGDPRPR IGVAIGDQIL DLSIIKHLFT) of Human Fumarylacetoacetate hydrolase (NP_000128)

  • 阳性对照
    • Fetal liver lysate.

性能

应用

Our Abpromise guarantee covers the use of ab83770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 46 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

靶标

  • 相关性Fumarylacetoacetate hydrolase is the last enzyme in the tyrosine catabolism pathway. FAH deficiency is associated with Type 1 hereditary tyrosinemia (HT). This is an autosomal recessive inborn error of metabolism that occurs in both an acute and a chronic form. Clinical characteristics of the acute form include hepatic failure and death in infancy, whereas children with the chronic form have renal tubular dysfunction and hypophosphatemic rickets, progressive liver disease with development of hepatocellular carcinoma. Dietary treatment with restriction of tyrosine and phenylalanine alleviates the rickets, but liver transplantation has so far been the only definite treatment.
  • 数据库链接
  • 别名
    • Beta diketonase antibody
    • FAA antibody
    • FAH antibody
    • Fumarylacetoacetase antibody
    • Fumarylacetoacetate antibody
    • Fumarylacetoacetate hydrolase antibody
    see all

Anti-Fumarylacetoacetate hydrolase antibody 图像

  • Immunohistochemistry with Human liver and mouse KO tissue
  • Immunohistochemistry with Human liver and mouse KO tissue
  • Anti-Fumarylacetoacetate hydrolase antibody (ab83770) at 1 µg/ml + Human fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 46 kDa
    Observed band size : 46 kDa
    Gel concentration 12%

Anti-Fumarylacetoacetate hydrolase antibody (ab83770)参考文献

ab83770 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (liver)
Loading amount 30 µg
Specification liver
Gel Running Conditions Reduced Denaturing (4-20% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Dec 13 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"