Fumarase Specific Activity Assay试剂盒(ab110043)

概述

  • 产品名称Fumarase Specific Activity Assay试剂盒
    参阅全部 Fumarase 试剂盒
  • 检测方法Colorimetric
  • 检测类型Enzyme activity
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Rat, Cow, Human
    不与反应: Mouse
  • 产品概述

    ab110043 (MS780) is a novel assay that uses a high affinity monoclonal capture antibody to selectively isolate fumarase (FH), which removes all other enzymes of the Krebs cycle and NAD+/NADH utilizing enzymes. After isolation and subsequent measurement of the enzyme's functional activity, the quantity of isolated FH is measured in the same well by adding a second monoclonal detector antibody, which is quantified using a colorimetric label (HRP). Both reactions take place in time-dependent manners proportional to the amount of enzyme captured in each well. By combining activity and quantity measurements, the enzyme's relative specific activity can be determined. Specific activity is useful for measuring up or down regulation of activity by site-specific modification or damage, and in response to specific inhibitors.

  • 说明

    ab110043 (MS780) is a novel assay that uses a high affinity monoclonal capture antibody to selectively isolate fumarase (FH), which removes all other enzymes of the Krebs cycle and NAD+/NADH utilizing enzymes. After isolation and subsequent measurement of the enzyme's functional activity, the quantity of isolated FH is measured in the same well by adding a second monoclonal detector antibody, which is quantified using a colorimetric label (HRP). Both reactions take place in time-dependent manners proportional to the amount of enzyme captured in each well. By combining activity and quantity measurements, the enzyme's relative specific activity can be determined. Specific activity is useful for measuring up or down regulation of activity by site-specific modification or damage, and in response to specific inhibitors.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

应用

Our Abpromise guarantee covers the use of ab110043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution.

Fumarase Specific Activity Assay Kit 图像

  • Example data obtained using the Fumarase Specific Activity Assay Kit (ab110043).
  • Principle of a Sandwich ELISA.

实验方案

Fumarase Specific Activity Assay Kit (ab110043)参考文献

ab110043 has not yet been referenced specifically in any publications.

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Thank you for contacting us and for bringing this error to our attention.

The units should all me in ug, not mg.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or informa...

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Thank you for contacting us.

I can confirm that ab110043 Fumarase Specific Activity Assay Kit does worki with Rat, Cow and Human samples. This product will not work with mouse.

I hope this information is helpful to you. Please...

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Thank you for contacting us.

The antibody used in this kit detects only human, rat and cow FH. It does not detect it in mouse samples. Therefore, we do not expect to see the activity from mouse samples in this immunocapture based activity as...

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I am still waiting for mouse reactivity data for the fumarase assay, ab110043. I did confirm, however, that the ATP synthase assay, ab109714, does come with 12 strips of 8 wells each, and not a standard solid microplate.

Thank you very much for your interest in our products.

I just sent you Proforma Invoice (quotation) #45550 for the 4 kits. This quote will be good until Sept 30th when the discount expires. When you're ready to place the order, just call us a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"