概述

  • 产品名称Anti-FUK抗体
    参阅全部 FUK 一抗
  • 描述
    兔多克隆抗体to FUK
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide derived from an internal region of human FUK.

  • 阳性对照
    • Extracts from insulin-treated COS7 cells. NIH-3T3 cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+) , 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab69555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 118 kDa).
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.

靶标

Anti-FUK antibody 图像

  • All lanes : Anti-FUK antibody (ab69555) at 1/500 dilution

    Lane 1 : Extracts from COS7 cells, treated with Insulin (0.01U/ml, 45mins)
    Lane 2 : Extracts from COS7 cells, treated with Insulin (0.01U/ml, 45mins) with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 118 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence analysis of FUK expression on NIH-3T3 cells, using ab 69555 at 1/500 dilution, with and without the immunising peptide.

Anti-FUK antibody (ab69555)参考文献

ab69555 has not yet been referenced specifically in any publications.

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