概述

  • 产品名称
  • 描述
    兔多克隆抗体to FUBP3
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Horse, Chicken, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between amino acids 522 - 572 of Human FUBP3 (NP_003925.1)

  • 阳性对照
    • HeLa, 293T or NIH 3T3 whole cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab108222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Predicted molecular weight: 62 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能
    May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression.
  • 组织特异性
    Detected in a number of cell lines.
  • 序列相似性
    Contains 4 KH domains.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Far upstream element (FUSE) binding protein 3 antibody
    • Far upstream element binding protein 3 antibody
    • Far upstream element-binding protein 3 antibody
    • FBP3 antibody
    • FLJ25229 antibody
    • FUBP 3 antibody
    • FUBP3 antibody
    • FUBP3_HUMAN antibody
    • FUSE binding protein 3 antibody
    • FUSE-binding protein 3 antibody
    • OTTHUMP00000022369 antibody
    see all

Anti-FUBP3 antibody 图像

  • All lanes : Anti-FUBP3 antibody (ab108222) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH 3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 62 kDa


    Exposure time : 3 minutes
  • Detection of FUBP3 by western blot of immunoprecipitate.
    ab108222 at 1 µg/ml staining FUBP3 in HeLa whole cell lysate immunoprecipitated using ab108222 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: chemiluminescence with an exposure time of 30 seconds.

Anti-FUBP3 antibody (ab108222)参考文献

ab108222 has not yet been referenced specifically in any publications.

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