概述

  • 产品名称Anti-FUBP1抗体
    参阅全部 FUBP1 一抗
  • 描述
    兔多克隆抗体to FUBP1
  • 经测试应用适用于: IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 540-589 (YYAHYYQQQA QPPPAAPAGA PTTTQTNGQG DQQNPAPAGQ VDYTKAWEEY) of human FUBP1 (NP_003893).

  • 阳性对照
    • HepG2 cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab83453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:1562500.

靶标

Anti-FUBP1 antibody 图像

  • Anti-FUBP1 antibody (ab83453) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 Cell Lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 68 kDa
    Observed band size : 68 kDa
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue labelling FUBP1 with ab83453 at 5.0µg/ml.

Anti-FUBP1 antibody (ab83453)参考文献

ab83453 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"