概述

  • 产品名称
  • 描述
    兔多克隆抗体to FUBP1
  • 经测试应用
    适用于: IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 540-589 (YYAHYYQQQA QPPPAAPAGA PTTTQTNGQG DQQNPAPAGQ VDYTKAWEEY) of human FUBP1 (NP_003893).

  • 阳性对照
    • HepG2 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab83453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:1562500.

靶标

  • 功能
    Regulates MYC expression by binding to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter. May act both as activator and repressor of transcription.
  • 序列相似性
    Contains 4 KH domains.
  • 翻译后修饰
    Ubiquitinated. This targets the protein for proteasome-mediated degradation.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DNA helicase V antibody
    • far upstream element (FUSE) binding protein 1 antibody
    • Far upstream element (FUSE) binding protein 4 antibody
    • Far upstream element binding protein 1 antibody
    • far upstream element binding protein antibody
    • Far upstream element-binding protein 1 antibody
    • FBP antibody
    • FUBP antibody
    • Fubp1 antibody
    • FUBP1_HUMAN antibody
    • Fubp4 antibody
    • FUSE binding protein 1 antibody
    • FUSE-binding protein 1 antibody
    • HDH V antibody
    see all

Anti-FUBP1 antibody 图像

  • Anti-FUBP1 antibody (ab83453) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 Cell Lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 68 kDa
    Observed band size : 68 kDa
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue labelling FUBP1 with ab83453 at 5.0µg/ml.

Anti-FUBP1 antibody (ab83453)参考文献

ab83453 has not yet been referenced specifically in any publications.

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