概述

  • 产品名称Anti-FTSJ3抗体
    参阅全部 FTSJ3 一抗
  • 描述
    兔多克隆抗体to FTSJ3
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 623-654 of Human FTSJ3 (NP_060117.3).

  • 阳性对照
    • MDA-MB231 cell line lysates. This antibody gave a positive result when used in the following methanol fixed cell lines: MCF-7

性能

应用

Our Abpromise guarantee covers the use of ab110884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 97 kDa.
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能Probable methyltransferase involved in the processing of the 34S pre-rRNA to 18S rRNA and in 40S ribosomal subunit formation.
  • 序列相似性Belongs to the methyltransferase superfamily. RlmE family. SPB1 subfamily.
  • 细胞定位Nucleus > nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2''-O-ribose RNA methyltransferase SPB1 homolog antibody
    • EPCS3 antibody
    • FLJ20062 antibody
    • FtsJ homolog 3 (E. coli) antibody
    • FTSJ3 antibody
    • pre-rRNA processing protein FTSJ3 antibody
    • Protein ftsJ homolog 3 antibody
    • Putative rRNA methyltransferase 3 antibody
    • SPB1 antibody
    • SPB1_HUMAN antibody
    see all

Anti-FTSJ3 antibody 图像

  • ICC/IF image of ab110884 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab110884 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-FTSJ3 antibody (ab110884) at 1/100 dilution + MDA-MB231 cell line lysates at 35 µg

    Predicted band size : 97 kDa

Anti-FTSJ3 antibody (ab110884)参考文献

ab110884 has not yet been referenced specifically in any publications.

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