使用敲除细胞株进行验证

Anti-FTO抗体(ab94482)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to FTO
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Sheep, Rabbit, Cow, Dog, Pig, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human FTO.

    (Peptide available as ab109634.)

  • 阳性对照
    • This antibody gave a positive signal in HepG2 whole cell lysate as well as the following tissue lysates: Human brain; Mouse hippocampus; Mouse brain.

性能

应用

Our Abpromise guarantee covers the use of ab94482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).

靶标

  • 功能
    Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation.
  • 组织特异性
    Ubiquitously expressed, with relatively high expression in adrenal glands and brain; especially in hypothalamus and pituitary.
  • 疾病相关
    Defects in FTO are the cause of growth retardation developmental delay coarse facies and early death (GRDDCFED) [MIM:612938]. The disease consists of a severe children multiple congenital anomaly syndrome with death by the age of 3 years. All affected individuals had postnatal growth retardation, microcephaly, severe psychomotor delay, functional brain deficits, and characteristic facial dysmorphism. In some patients, structural brain malformations, cardiac defects, genital anomalies, and cleft palate were also observed.
  • 序列相似性
    Belongs to the fto family.
  • 结构域
    The 3D-structure of the Fe2OG dioxygenase domain is similar to that of the Fe2OG dioxygenase domain found in the bacterial DNA repair dioxygenase alkB and its mammalian orthologs, but sequence similarity is very low. As a consequence, the domain is not detected by protein signature databases.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AlkB homolog 9 antibody
    • ALKBH9 antibody
    • Alpha-ketoglutarate-dependent dioxygenase FTO antibody
    • AW743446 antibody
    • Fat mass and obesity-associated protein antibody
    • FATSO, MOUSE, HOMOLOG OF antibody
    • Fto antibody
    • FTO_HUMAN antibody
    • GDFD antibody
    • KIAA1752 antibody
    • mKIAA1752 antibody
    • Protein fatso antibody
    see all

Anti-FTO antibody 图像



  • Predicted band size : 58 kDa

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: FTO knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HEK293 whole cell lysate (20 µg)
    Lane 4: MOLT4 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab94482 observed at 58 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab94482 was shown to recognize FTO when FTO knockout samples were used, along with additional cross-reactive bands. Wild-type and FTO knockout samples were subjected to SDS-PAGE.  Ab94482 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-FTO antibody (ab94482) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Mouse Hippocampus Tissue Lysate
    Lane 3 : Brain (Mouse) Tissue Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
    Additional bands at : 20 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

实验方案

Anti-FTO antibody (ab94482)参考文献

ab94482 has not yet been referenced specifically in any publications.

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