Anti-FTO抗体(ab77547)

概述

  • 产品名称Anti-FTO抗体
    参阅全部 FTO 一抗
  • 描述
    山羊多克隆抗体to FTO
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Dog, Pig
  • 免疫原

    Synthetic peptide:

    QQKPDCRPYWEKDD

    , corresponding to internal sequence amino acids 464-477 of Mouse FTO (NP_036066.2).

  • 阳性对照
    • Human Pancreas lysate.

性能

应用

Our Abpromise guarantee covers the use of ab77547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明Peptide ELISA: Antibody detection limit dilution 1/128000.
    WB: Use at a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation.
    • 组织特异性Ubiquitously expressed, with relatively high expression in adrenal glands and brain; especially in hypothalamus and pituitary.
    • 疾病相关Defects in FTO are the cause of growth retardation developmental delay coarse facies and early death (GRDDCFED) [MIM:612938]. The disease consists of a severe children multiple congenital anomaly syndrome with death by the age of 3 years. All affected individuals had postnatal growth retardation, microcephaly, severe psychomotor delay, functional brain deficits, and characteristic facial dysmorphism. In some patients, structural brain malformations, cardiac defects, genital anomalies, and cleft palate were also observed.
    • 序列相似性Belongs to the fto family.
    • 结构域The 3D-structure of the Fe2OG dioxygenase domain is similar to that of the Fe2OG dioxygenase domain found in the bacterial DNA repair dioxygenase alkB and its mammalian orthologs, but sequence similarity is very low. As a consequence, the domain is not detected by protein signature databases.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AlkB homolog 9 antibody
      • ALKBH9 antibody
      • Alpha-ketoglutarate-dependent dioxygenase FTO antibody
      • AW743446 antibody
      • Fat mass and obesity-associated protein antibody
      • FATSO, MOUSE, HOMOLOG OF antibody
      • Fto antibody
      • FTO_HUMAN antibody
      • GDFD antibody
      • KIAA1752 antibody
      • mKIAA1752 antibody
      • Protein fatso antibody
      see all

    Anti-FTO antibody 图像

    • Anti-FTO antibody (ab77547) at 0.03 µg/ml + Human Pancreas lysate (in RIPA buffer) at 35 µg
      Developed using the ECL technique

      Predicted band size : 58 kDa
      Observed band size : 58 kDa

    Anti-FTO antibody (ab77547)参考文献

    ab77547 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting Abcam.

    For ab77547 we recommend blocking in 3% milk, along with incubating both the primary and secondary antibody in 3% milk also.

    Please let us know if there is anything else I can help you with.

    Thank you for contacting us. The antibody was blocked in 3% milk in TBST for 1 hour at room temperature.



    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"