概述

  • 产品名称Anti-FTO抗体[5-2H10]
    参阅全部 FTO 一抗
  • 描述
    小鼠单克隆抗体[5-2H10] to FTO
  • 经测试应用适用于: WB, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Sheep, Horse, Cow, Dog, Non Human Primates
  • 免疫原

    Synthetic peptide from Human FTO.

  • 阳性对照
    • WB: Rat testes nuclear lysate ICC/IF: Neuronal progenitor cells

性能

应用

Our Abpromise guarantee covers the use of ab92821 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
ICC/IF 1/100.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation.
  • 组织特异性Ubiquitously expressed, with relatively high expression in adrenal glands and brain; especially in hypothalamus and pituitary.
  • 疾病相关Defects in FTO are the cause of growth retardation developmental delay coarse facies and early death (GRDDCFED) [MIM:612938]. The disease consists of a severe children multiple congenital anomaly syndrome with death by the age of 3 years. All affected individuals had postnatal growth retardation, microcephaly, severe psychomotor delay, functional brain deficits, and characteristic facial dysmorphism. In some patients, structural brain malformations, cardiac defects, genital anomalies, and cleft palate were also observed.
  • 序列相似性Belongs to the fto family.
  • 结构域The 3D-structure of the Fe2OG dioxygenase domain is similar to that of the Fe2OG dioxygenase domain found in the bacterial DNA repair dioxygenase alkB and its mammalian orthologs, but sequence similarity is very low. As a consequence, the domain is not detected by protein signature databases.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AlkB homolog 9 antibody
    • ALKBH9 antibody
    • Alpha-ketoglutarate-dependent dioxygenase FTO antibody
    • AW743446 antibody
    • Fat mass and obesity-associated protein antibody
    • FATSO, MOUSE, HOMOLOG OF antibody
    • Fto antibody
    • FTO_HUMAN antibody
    • GDFD antibody
    • KIAA1752 antibody
    • mKIAA1752 antibody
    • Protein fatso antibody
    see all

Anti-FTO antibody [5-2H10] 图像

  • Anti-FTO antibody [5-2H10] (ab92821) at 1/1000 dilution + Rat testes lysate

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
  • ICC/IF image of ab92821 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92821, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Overlay histogram showing SH-SY5Y cells stained with ab92821 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92821, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-FTO antibody [5-2H10] (ab92821)参考文献

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